多壁碳纳米管修饰玻碳电极电致化学发光测奈福泮  

Electrochemiluminescence determination of nefopam by multi-wall carbon nanotube modified glassy carbon electrode

作　　者：李学暖 李天昊 张嘉琳 黎孔月 蒋靖超 程昊[1,2,3] LI Xuenuan;LI Tianhao;ZHANG Jialin;LI Kongyue;JIANG Jingchao;CHENG Hao(School of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Guangxi Key Laboratory of Green Processing of Sugar Resources(Guangxi University of Science and Technology),Liuzhou 545006,China;Province and Ministry Co-sponsored Collaborative Innovation Center of Sugarcane and Sugar Industry,Nanning 530004,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西糖资源绿色加工重点实验室(广西科技大学),广西柳州545006 [3]蔗糖产业省部共建协同创新中心,广西南宁530004

出　　处：《广西科技大学学报》2020年第4期124-131,共8页Journal of Guangxi University of Science and Technology

基　　金：2019年广西糖资源绿色加工重点实验室开放课题(GXTZY201701);广西高等学校高水平创新团队及卓越学者计划(桂教人〔2014〕7号);广西高校大学生创新创业计划项目(201910594075,201910594076)资助。

摘　　要：借助多壁碳纳米管修饰电极对奈福泮有良好的电催化活性,以多壁碳纳米管修饰玻碳电极为工作电极,铂丝电极为对电极,饱和氯化银电极为参比电极形成三电极体系,在支持电解质为pH=7的磷酸盐缓冲液下研究了奈福泮-联吡啶钌体系[Ru(bpy)3^2+]的电化学发光行为,建立了多壁碳纳米管(MWCNTs)修饰剂固载联吡啶钌体系[Ru(bpy)3^2+]测定盐酸奈福泮的电致化学发光分析方法.研究结果表明:在0.1 mol/L、pH为7.5的磷酸缓冲溶液中,当修饰量为3μL,钌的浓度为0.2 mmol/L,扫描速率为100 mV/s时,ECL的峰高与奈福泮的浓度在1×10^-5~1×10^-4 mol/L内成良好线性关系,线性方程为I=908.95×10^5x-323.88(R2=0.9951),检出限为4×10^-8 mol/L(S/N=3),RSD为1.57%,并测得其回收率为97.85%~103.01%.该方法具有较高的选择性和灵敏度,样品处理简单快速,用于奈福泮的测定,结果满意.The multi-walled carbon nanotube modified electrode has good electrocatalytic activity for Nefopam. With the multi-walled carbon nanotube modified glassy carbon electrode as the working electrode, platinum wire electrode as counter electrode, Ag/Agcl saturated silver chloride electrode as reference electrode to build a three-electrode system, the electrochemical luminescence behavior of nefopam-tridipyridinium ruthenium system Ru( bpy)3^2+was investigated in phosphate buffer supported with p H=7 electrolyte. And an electrochemiluminescence analysis method for the determination of nefopam hydrochloride lti-walled carbon nanotube(MWCNTs) modifier-immobilized bipyridylruthenium system Ru( bpy)3^2+was established. The results show that, under the phosphoric acid buffer solution of0.1 mol/L with pH 7.5, the concentration of the modified amount is 3 μL, the concentration of Ru( bpy) 3^2+is 0.2 mmol/L and the scan rate is 100 mV/s. The method mentioned above shows a linear relationship between the peak of ECL and the concentration of nefopam in the range of 1×10^-5-1×10^-4 mol/L, the linear equation is I=908.95×105 x-323.88(R2=0.995 1), the low detection limit is 4×10^-8 mol/L. The RSD for 3 times determination of nefopam solution of 4×10^-8 mol/L is 1.57%. The recoveries of the sample is 97.85%-103.01%. The ECL method has high selectivity and sensitivity, and the sample treatment is simple and rapid. In addition, the method has been applied to the determination of nefopam with satisfactory results.

关 键 词：联吡啶钌 电致化学发光 奈福泮 玻碳电极 

分 类 号：O657.3[理学—分析化学]