IL-10和TREM-1水平与急性胰腺炎大鼠病情进展的相关研究  

Correlation between IL-10 and TREM-1 levels and disease progression in rats with acute pancreatitis

作　　者：高晓洁 马德超 王树成[1] GAO Xiaojie;MA Dechao;WANG Shucheng(Wuqing People Hospital,Tianjin 301700,China)

机构地区：[1]天津市武清区人民医院,天津301700

出　　处：《河南大学学报（医学版）》2020年第5期322-326,共5页Journal of Henan University:Medical Science

基　　金：天津市武清区科技发展计划项目(WQKJ201842)。

摘　　要：[目的]探讨白细胞介素10(IL-10)和髓样细胞触发性受体-1(TREM-1)在不同病情的急性胰腺炎大鼠模型中的含量变化,判断IL-10与TREM-1水平变化是否与急性胰腺炎发病、病情严重程度及发病时间具有相关性。[方法]50只大鼠随机分为对照组(10只)、急性轻型胰腺炎12 h组(10只)、急性轻型胰腺炎24 h组(10只)、急性重症胰腺炎12 h组(10只)、急性重症胰腺炎24 h组(10只);酶联反应吸附法(Elisa法)检测各组大鼠血清中IL-10、TREM-1含量;生化法检测各组大鼠血清淀粉酶水平。HE染色法检测各组胰腺组织的病理学变化。[结果]急性重症胰腺炎24 h组大鼠血清淀粉酶水平和IL-10、TREM-1含量较其他组明显升高(P<0.05)。急性胰腺炎病情严重程度(病理学评分)与IL-10、TREM-1含量及血清淀粉酶水平呈正相关(P<0.05)。[结论]IL-10和TREM-1的高表达可能是急性重症胰腺炎病情进展和影响预后的因素之一。[Objective]To investigate the changes of interleukin-10(IL-10) and myeloid cell-triggered receptor-1(TREM-1) in rat models with different conditions of acute pancreatitis, and to determine whether the changes in il-10 and trem-1 levels were correlated with the onset, severity and duration of acute pancreatitis. [Methods]Fifty rats were randomly divided into control group(10), acute mild pancreatitis 12 hour group(10), acute mild pancreatitis 24 hour group(10), acute severe pancreatitis 12 hour group(10), acute severe pancreatitis 24 hour group(10). The levels of IL-10 and TREM-1 in serum were determined by Elisa.Serum amylase levels were measured by biochemical method. Pathological changes of pancreatic tissues in each group were detected by HE staining. [Results]The levels of serum amylase and IL-10 and TREM-1 in the 24 hour acute severe pancreatitis group were significantly higher than those in the other groups(P<0.05). [Conclusion]The high expression of IL-10 and TREM-1 may be one of the factors influencing the progression and prognosis of acute severe pancreatitis.

关 键 词：胰腺炎 IL-10 TREM-1 

分 类 号：R576[医药卫生—消化系统]