HPLC-RID测定发酵虫草菌粉(Cs-4)中甘露醇的含量  

Content Determination of Mannitol in Fermentative Cordycepic Fungal Powder(CS-4)by HPLC-RID

作　　者：赵鹏 杨明 ZHAO Peng;YANG Ming(Jiangxi Jemincare Group CO.,LTD.,Nanchang 330096,China)

机构地区：[1]江西济民可信集团有限公司,南昌330096

出　　处：《药品评价》2020年第14期56-58,共3页Drug Evaluation

基　　金：中药口服制剂先进制药与信息化技术融合示范研究(2018ZX092010-001-001)。

摘　　要：目的:建立测定发酵虫草菌粉(Cs-4)中甘露醇含量的高效液相色谱-示差折光检测器法(HPLC-RID)。方法:采用Carbomix Ca-NP 5∶8 %色谱柱(7.8 mm × 300 mm,5 μm),以乙腈-水(10∶90)为流动相;流速为0.5 mL/min;柱温为80 ℃;示差折光检测器温度为55 ℃。结果:甘露醇质量浓度在0.099~3.955 mg/mL范围内线性关系良好(r=0.999 7);方法的精密度、稳定性及重复性良好,RSD均小于2.0 %;平均回收率为96.0 %(RSD=1.67 %)。结论:经方法学验证,本法可用于发酵虫草菌粉(Cs-4)中甘露醇的含量测定,为发酵虫草菌粉(Cs-4)的全面质量控制提供科学依据。Objective:To establish an HPLC-RID method for the determination of mannitol in fermentative cordycepic fungal powder(CS-4).Methods:HPLC-RID experiments was performed on Carbomix Ca-NP 5∶8%column(7.8 mm×300 mm,5μm).The mobile phase consisted of acetonitrile-water(10∶90).The flow rate of 0.5 mL/min.The column temperature was 80℃and detector temperature was 55℃.Results:Mannitol show a good liner relationship within the range of 0.099~3.955 mg/mL,respectively(r=0.9997).Good precision,stability and reproducibility was achieved with the RSDs below 2.0%.The average recovery of mannitol 96.0%(RSD=1.67%).Conclusion:Through methodology validation,This method can be used in determination of mannitol in fermentative cordycepic fungal powder(CS-4),and it provide a scientific basis for the comprehensive quality control of fermentative cordycepic fungal powder(CS-4).

关 键 词：发酵虫草菌粉(Cs-4) HPLC-RID 甘露醇 含量测定 

分 类 号：R917[医药卫生—药物分析学]