同源血浆及血清中蓝舌病病毒抗体ELISA检测结果比较  

Comparison of the Test Results of Antibody against Bluetongue Virus in Homologous Plasma and Serum by ELISA

作　　者：段莹亮 李占鸿 廖德芳[1] 李乐[1] Duan Yingliang;Li Zhanhong;Liao Defang;Li Le(Yunnan Key Laboratory of Tropical and Subtropical Animal Virus Diseases,Yunnan Animal Science and Veterinary Institute,Kunming,Yunnan 650224,China)

机构地区：[1]云南省畜牧兽医科学院,云南省热带亚热带动物病毒病重点实验室,云南昆明650224

出　　处：《中国动物检疫》2020年第11期91-95,111,共6页China Animal Health Inspection

基　　金：国家重点研发计划项目(2017YFD0502304-5)。

摘　　要：为简化动物血液样品中蓝舌病病毒(BTV)检测及研究方法,采用2种酶联免疫吸附试验(ELISA),对采自云南省德宏州芒市一家肉牛养殖场的30对牛同源血浆及血清样品进行BTV总抗体及VP7抗体检测。BTV总抗体ELISA检测结果显示,同源血浆与血清之间的阳/阴性结果一致率为100%,ΔPI在±5%以内的样品占87%;VP7抗体ELISA检测结果显示,同源血浆与血清之间的阳/阴性结果一致率为93%,ΔOD在±0.05范围内的样品占87%。结果表明:利用ELISA检测动物血样中BTV抗体时,血浆样品可替代血清样品;血浆样品的溶血程度不影响检测结果,但粗制血浆中的非溶解性杂质可能影响检测结果,需要使用离心除杂后的血浆样品。In order to simplify the test and research method of bluetongue virus(BTV)in animal blood samples,30 pairs of bovine homologous plasma and serum samples collected from a beef cattle farm in Mang City,Dehong Prefecture,Yunnan Province were tested for antibodies against BTV and VP7 protein.The results showed that,for the detection of total antibodies of BTV,the consistency rate of the positive/negative results of homologous plasma and serum was 100%,and samples withΔPI less than±5%accounted for 87%;as tested by VP7 Ab ELISA kit,the consistency rate of the positive/negative results of homologous plasma and serum was 93%,and samples withΔOD less than±5%accounted for 87%.Therefore,serum samples might be replaced by plasma samples when the antibody against BTV in animal blood was tested by ELISA,and the test results could not be affected by the hemolysis level of plasma samples rather than the insoluble impurities in crude plasma which should be removed by centrifugation before being used.

关 键 词：蓝舌病病毒 血浆 血清 酶联免疫吸附试验 

分 类 号：S852.65[农业科学—基础兽医学]