龙胆泻肝汤激活Bax/Caspase-3通路并诱导人角质形成细胞凋亡  

Longdanxiegan decoction activates Bax/Caspase-3 pathway and induces the apoptosis of HaCaT cells

作　　者：杨冠群 郭春 魏海峰[2] 周煜 周曙杰 梁晨希 吴国境 许博涵 邹永新 张晓杰[3] 孙淑娜[3] YANG Guanqun;GUO Chun;WEI Haifeng;ZHOU Yu;ZHOU Shujie;LIANG Chenxi;WU Guojing;XU Bohan;ZOU Yongxin;ZHANG Xiaojie;SUN Shuna(Department of Genetics,School of Basic Medical Sciences,Cheeloo College of Medicine,Shandong University,Jinan 250012,China;Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Department of Dermatology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011,China)

机构地区：[1]山东大学齐鲁医学院基础医学院遗传学系,山东济南250012 [2]山东中医药大学,山东济南250355 [3]山东中医药大学附属医院皮肤科,山东济南250011

出　　处：《中国麻风皮肤病杂志》2020年第11期663-666,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金(编号:81804104,31671427)。

摘　　要：目的:明确龙胆泻肝汤对人角质形成细胞株HaCaT细胞凋亡的影响。方法:Tunel法检测龙胆泻肝汤对HaCaT细胞凋亡的影响;DAPI染色后荧光显微镜观察HaCaT细胞凋亡形态变化;Western blot检测分析HaCaT细胞凋亡相关蛋白表达。结果:龙胆泻肝汤处理组HaCaT细胞形态发生了明显变化,凋亡比例明显高于未处理细胞组。龙胆泻肝汤作用于HaCaT细胞后活化的caspase-3和Bax的表达明显上调, Bcl-2蛋白表达下降。caspase-3特异性抑制剂Z-DEVD-FMK预处理后可降低龙胆泻肝汤诱导的细胞凋亡。结论:龙胆泻肝汤能明显诱导人角质形成细胞的凋亡,其机制可能与下调Bcl-2的表达,增高Bax和caspase-3的表达有关。Objective:To determine the role of Longdanxiegan decoction in the apoptosis of HaCaT cells.Methods:The effect of Longdanxiegan decoction on the apoptosis of HaCaT cells was detected by Tunel method.The apoptosis of HaCaT cells was detected by fluorescence microscopy after DAPI staining.The effect of HaCaT cells on the expression of apoptosis-related proteins caspase-3,Bcl-2 and Bax was detected by Western blot.Results:The abnormal cells shape rate and the apoptosis rate were higher in the Longdanxiegan decoction group than in the untreated group.Longdanxiegan decoction significantly down-regulated the expression level of Bcl-2 and up-regulated Bax,and actived caspase in HaCaT cells.The cell apoptosis caused by Longdanxiegan decoction decreased in the cells pretreated by Z-DEVD-FMK(inhibitor of caspase-3).Conclusion:Longdanxiegan decoction can significantly induce apoptosis of human keratinocytes,which may be through down-regulation of Bcl-2 and up-regulation of caspase-3 or Bax.

关 键 词：龙胆泻肝汤 HACAT细胞 银屑病 细胞凋亡 

分 类 号：R285[医药卫生—中药学]