一步水热法制备氮掺杂碳点荧光探针及对硝基苯酚的检测  

Nitrogen-doped carbon dots by one-step hydrothermal method as a fluorescent probe to detect p-nitrophenol

作　　者：王宁[1] 石璟 张梦婷 肖虹[3] 李宁[3] 杨学芳 卞伟[1] WANG Ning;SHI Jing;ZHANG Meng-ting;XIAO Hong;LI Ning;YANG Xue-fang;BIAN Wei(School of Basic Medical Science,Shanxi Medical University,Taiyuan 030001,China;School of Graduate,Shanxi Medical University,Taiyuan 030001,China;The First Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学基础医学院,山西太原030001 [2]山西医科大学研究生学院,山西太原030001 [3]山西医科大学第一医院,山西太原030001

出　　处：《化学研究与应用》2020年第10期1772-1781,共10页Chemical Research and Application

基　　金：山西省自然科学基金项目(201901D111210)资助;山西医科大学博士启动基金项目(03201514)资助;山西医科大学创新基金项目(01201312)资助。

摘　　要：本实验采用一步水热法,以碧桃为原料制备氮掺杂碳点(N-CDs),并使用透射电子显微镜(TEM)、X射线光电子能谱(XPS)、傅里叶转换红外光谱(FT-IR)等技术对其形貌和化学结构进行表征,用荧光光谱和紫外可见吸收光谱考察了其光学性质。N-CDs的荧光量子产率为21.39%。基于对硝基苯酚(p-NP)可以有效猝灭N-CDs的荧光,建立了一种快速灵敏检测p-NP的荧光方法。探讨了p-NP与N-CDs两者间相互作用的猝灭机理主要为静态猝灭。在最佳实验条件下,p-NP浓度在6.67×10-7 M^9.86×10-5 M范围内与N-CDs的荧光强度呈现良好的线性关系,检出限为0.045μM。将该方法用于环境水样及从事化学行业相关人员的生物样品中p-NP的检测,加标回收率分别为99.8%~104.9%和97.7%~104.4%。In this manuscript,one-step hydrothermal method was used to prepare nitrogen-doped carbon dots(N-CDs)using Flowering Peach as raw material.The morphology and chemical structure of N-CDs were characterized by TEM、XRD、XPS and FT-IR,and their optical properties were investigated by fluorescence spectra and ultraviolet-visible absorption spectroscopy.The fluorescence quantum yield of N-CDs was 21.39%.Based on the fact that p-nitrophenol(p-NP)can effectively quench the fluorescence of N-CDs,a fast and sensitive fluorescence probe for detecting the p-NP was established.The quenching mechanism of the interaction between p-NP and N-CDs was mainly static quenching.Under the best experimental conditions,the concentration of p-NP in the range of 6.67×10^-7 M^9.86×10^-5 M decipted the good linear relationship with the fluorescence intensity of N-CDs and the limit of detection was 0.045μM.The probe was also applied to detect p-NP in water samples and in biological samples of people working in the chemical industry with the recovery rates of 99.8%~104.9%and 97.7%~104.4%,respectively.

关 键 词：氮掺杂碳点 对硝基苯酚 一步水热法 荧光猝灭 

分 类 号：O657.3[理学—分析化学]