1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲的合成及荧光猝灭法测定海产品中的微量汞  

Synthesis of 1-(4-dimethyl aminobenzylidene)-4-methyl-thiourea and fluorescene quenching determination of trace mercury in sea products

作　　者：张夏红[1,2] 董雁 荀瑞芝[1] 俞芸 ZHANG Xia-hong;DONG Yan;XUN Rui-zhi;YU Yun(Department of Chemistry and Material Engineering,Longyan University,Longyan 364012,China;Fujian Provincial colleges and University Engineering Research Center of Solide Waste Resource Utilization,Longyan 364012,China)

机构地区：[1]龙岩学院化学与材料学院,福建龙岩364012 [2]固体废弃物资源利用福建省高校工程研究中心,福建龙岩364012

出　　处：《化学研究与应用》2020年第10期1839-1846,共8页Chemical Research and Application

基　　金：福建省教育厅中青年教育科研项目(JA15507)资助;福建省重点学科项目资助;龙岩市科技局重点计划项目(2015LY25)资助。

摘　　要：合成了荧光试剂1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲(DMAT),其水溶液在448 nm处发射强荧光,通过研究DMAT在水溶液中对重金属离子的识别作用,发现DMAT对汞离子的识别具有高选择性、高灵敏性,且DMAT和汞(Ⅱ)形成配位比为1∶1的稳定络合物,产生荧光猝灭现象。据此建立了测定汞(Ⅱ)含量的1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲荧光分光光度法,其线性范围是0~9.9×10^-6 moL·L^-1,检出限为6.3×10^-8 moL·L^-1。该法用于海产品花蛤和虾头中微量汞的测定,方法简单,结果满意。A new fluorescent reagent 1-(4-dimethylaminobenzylidene)-4-methyl-thiourea(DMAT)was synthesized and exhibited excellent fluorescence emission at 448 nm in aqueous solution.Responses to other metal ions present in the solution,DMAT displayed extremely high sensitivity and selectivity to Hg(Ⅱ)in the water media.A stable complex was produced between DMAT and mercury(Ⅱ)with ratio of 1∶1,and the fluorescence of DMAT was quenched.Under optimal conditions,its response was linearly proportional to the concentration of Hg ions in a range from 0 to 9.9 × 10^-6 mol·L^-1 with a detection limit of 6.3×10^-8 mol·L^-1.The proposed method was successfully applied to the direct determination of Hg(Ⅱ)in clam and shrimp samples with satisfactory results.

关 键 词：1-(4-二甲氨基苯亚甲基氨基)-4-甲基硫脲 荧光猝灭 汞含量 

分 类 号：O657.3[理学—分析化学]