Xueshuantong for Injection(Lyophilized,注射用血栓通)Alleviates Streptozotocin-Induced Diabetic Retinopathy in Rats  

作　　者：LI Rui-lin WANG Jin-xin CHAI Li-juan GUO Hong WANG Hong CHEN Lu HU Li-min WANG Shao-xia 

机构地区：[1]Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin(300193),China [2]Key Laboratory of Pharmacology of Traditional Chinese Medical Formula,Ministry of Education,Tianjin University of Traditional Chinese Medicine,Tianjin(300193),China [3]School of Integrative Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin(300193),China

出　　处：《Chinese Journal of Integrative Medicine》2020年第11期825-832,共8页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81573644);Major New Drugs Innovation and Development(No.2012ZX09101201-004);Guangxi Provincial Department of Science and Technology(No.14125008-2-5);and Tianjin 131 Innovative Team Project and Training Program Foundation for Innovative Research Team of Higher Education in Tianjin(No.TD 13-5050)。

摘　　要：Objective To investigate the ameliorate effect and underlying mechanism of Xueshuantong for Injection(Lyophilized,注射用血栓通,XST)in streptozocin(STZ)-induced diabetic retinopathy(DR)rats.Methods Diabetes mellitus(DM)model was induced by intraperitoneal(i.p.)injection of STZ(60 mg/kg)in Sprague-Dawley rats.Diabetic rats were randomized into 3 groups(n=10)according to a random number table,including DM,XST50 and XST100 groups.XST treatment groups were daily i.p.injected with 50 or 100 mg/kg XST for 60 days,respectively.The control and DM groups were given i.p.injection with saline.Blood glucose level and body weight were recorded every week.Histological changes in the retina tissues were observed with hematoxylin-eosin staining.Apoptosis and inflammation related factors,including cleaved caspase-3,glial fifibrillary acidic protein(GFAP),tumor necrosis factor-α(TNF-α)and intercellular cell adhesion molecule-1(ICAM-1)were detected by Western blot or real-time polymerase chain reaction.Then,the levels of advanced glycation end product(AGE)and its receptor(RAGE)were investigated.Tight junctions proteins(Zonula occludens-1(ZO-1),Occludin and Claudin-5)of blood-retinal barrier were detected by Western blot.The levels of retinal fifibrosis,transforming growth factor-β1(TGF-β1)-Smad2/3 signaling pathway were evaluated at last.Results There was no signifificant difference in the body weight and blood glucose level between XST and DM groups(P>0.05).Compared with the DM group,XST treatment signifificantly increased the retinal thickness of rats(P<0.05 or P<0.01),and suppressed cleaved caspase-3 expression(P<0.01).XST increased the protein expressions of ZO-1,Occludin and Claudin-5 and decreased the mRNA expressions of matrix metalloproteinase 2(MMP-2)and MMP-9(P<0.05 or P<0.01).Moreover,XST signifificantly reduced the productions of AGE and RAGE proteins in the retina of rats(P<0.05 or P<0.01),suppressed the over-expression of TNF-α,and decreased the elevated level of ICAM-1 in retina of rats(P<0.05 or P<0.01).XS

关 键 词：Xueshuantong for Injection(Lyophilized) diabetic retinopathy Panax notoginseng saponin transforming growth factor-βp/Smad2/3 signaling pathway Chinese medicine 

分 类 号：R285.5[医药卫生—中药学]