枸杞多糖对DC2.4细胞增殖、抗原吞噬及成熟的影响  

Effects of Lycium Barbarum Polysaccharides on the Proliferation,Antigenic Phagocytosis and Maturation of DC2.4

作　　者：张炜 万巧凤 马锐 黄菱 王丽 ZHANG Wei;WAN Qiaofeng;MA Rui;HUANG Ling;WANG Li(Department of Pathogen Biology and Medical Immunology,School of Basic Medicine,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学基础医学院病原生物学与医学免疫学系,银川750004

出　　处：《宁夏医科大学学报》2020年第10期979-982,共4页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金(NZ16081)。

摘　　要：目的研究枸杞多糖(lycium barbarum polysaccharides,LBP)对未成熟树突状细胞DC2.4增殖、吞噬抗原及成熟的影响,为进一步阐释LBP抗炎、免疫调节作用的细胞分子机制提供基础。方法(1)采用CCK-8法测定高、中、低浓度LBP(分别对应LBP终浓度为200μg·mL^-1、100μg·mL^-1和20μg·mL^-1)对DC2.4细胞增殖的影响;(2)采用流式细胞仪检测高、中、低浓度LBP对DC2.4细胞吞噬卵清蛋白(ovalbumin,OVA)的影响;(3)采用流式细胞仪检测LBP、脂多糖(lipopolysaccharide,LPS)及LBP+LPS对DC2.4细胞成熟标志分子CD86表达的影响。结果(1)高、中浓度LBP在12 h、24 h及48 h对DC2.4细胞的增殖均无明显促进作用,低浓度LBP在24 h及48 h均能促进DC2.4细胞增殖(P均<0.05)。(2)高、中、低浓度LBP均可促进DC2.4细胞吞噬抗原,以100μg·mL^-1 LBP效果最佳。(3)LPS组和LBP组CD86的表达增加(P<0.01),而LPS+LBP组CD86表达低于LPS组(P<0.05)。结论LBP具有维持DC2.4细胞数量稳态的特性,可促进DC2.4细胞吞噬抗原,并具有适度抑制刺激树突状细胞成熟的作用。Objective To study the effects of lycium barbarum polysaccharides(LBP)on the proliferation,antigen phagocytosis and maturation of immature dendritic cells DC2.4,and investigate the underlying cellular and molecular mechanisms of the anti-inflammatory and immunomodulatory effects induced by LBP.Methods(1)CCK-8 method was used to determine the effects of high,medium and low concentrations of LBP(corresponding final concentrations of LBP are 200,100 and 20μg·mL^-1,respectively)on the proliferation of DC2.4.(2)The effects of high,medium and low concentrations of LBP on the phagocytosis of ovalbumin antigen(OVA)were detected by flow cytometry.(3)The effect of LBP,lipopolysaccharide(LPS),as well as LBP and LPS on the expression of cell maturation marker CD86 of DC2.4 was detected by flow cytometry.Results(1)High concentration and medium concentration of LBP did not significantly promote proliferation of DC2.4 cells at 12 h,24 h and 48 h,while low concentration of LBP significantly promoted proliferation of DC2.4 cells at 24 h and 48 h(P all<0.05).(2)High,medium and low concentrations of LBP could promote antigen phagocytosis of DC2.4 cells,the best effect was induced by 100μg·mL^-1LBP.(3)The expression of CD86 in LPS group and LBP group increased significantly(P<0.01),while that in LPS plus LBP group decreased significantly(P<0.05).Conclusion LBP has the ability to maintain the homeostasis of the number of DC2.4 cells,promote the antigen phagocytosis of DC2.4 cells,and moderately inhibit the maturation of dendritic cells induced by the inflammatory stimulator.

关 键 词：枸杞多糖 DC2.4 脂多糖 CD86 流式细胞术 

分 类 号：R285.5[医药卫生—中药学]