产地加工炮制一体化与传统大黄饮片化学成分的比较研究  

Comparative study on the chemical composition between integral processing and traditional processing of rhubarb pieces

作　　者：辛二旦 司昕蕾 边甜甜[1] 牛江涛[1] 张育贵 张淑娟 李越峰[1] XIN Erdan;SI Xinlei;BIAN Tiantian;NIU Jiangtao;ZHANG Yugui;ZHANG Shujuan;LI Yuefeng(Key Laboratory of Standard and Quality of Chinese Medicine Research of Gansu Province,University of Traditional Chinese Medicine of Gansu,Lanzhou,Gansu,730000 P.R.China)

机构地区：[1]甘肃中医药大学甘肃省中药质量与标准研究重点实验室,甘肃兰州730000

出　　处：《华西药学杂志》2020年第5期505-508,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81960713);自然科学基金创新基地和人才计划项目(18JR3RA197);甘肃省中药质量与标准研究重点实验室开放基金(ZYZL18-008);甘肃中医药大学研究生创新基金(CX2019-16)。

摘　　要：目的比较产地加工炮制一体化与传统大黄饮片的化学成分含量,探讨产地加工炮制一体化大黄饮片的优势。方法采用HPLC同时测定产地加工炮制一体化大黄饮片与传统大黄饮片中5种游离蒽醌(芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚)及6种结合蒽醌(芦荟大黄素-8-O-葡萄糖苷、大黄酸-8-O-葡萄糖苷、大黄素-1-O-葡萄糖苷、大黄酚-8-O-葡萄糖苷、大黄素-8-O-葡萄糖苷、大黄素甲醚-8-O-葡萄糖苷)的含量,对比分析产地加工炮制一体化与传统加工对大黄饮片化学成分含量的影响。结果产地加工炮制一体化大黄饮片中5种游离蒽醌及大黄酸-8-O-葡萄糖苷、大黄素-1-O-葡萄糖苷、大黄酚-8-O-葡萄糖苷和大黄素甲醚-8-O-葡萄糖苷的含量显著高于传统加工品;芦荟大黄素-8-O-葡萄糖苷和大黄素-8-O-葡萄糖苷的含量显著低于传统加工品。结论产地加工炮制一体能有效减少化学成分流失,保证大黄饮片的质量。OBJECTIVE To compare the chemical compositions between integral processing and traditional processing of rhubarb pieces,and to explore the advantages of integral processing rhubarb pieces.METHODS HPLC was adopted to simultaneously determine the contents of five free anthraquinones(aloe-emodin,rhein,emodin,chrysophanol and physcion)and six bound anthraquinones(aloe-emodin-8-O-glucopyranoside,rhein-8-O-glucopyranoside,emodin-1-glucoside,chrysophanol-8-glucoside,emodin-8-glucoside and physcion-8-glucoside)in inintegral processing and traditional processing of hubarb pieces.RESULTS The content of five free anthraquinones and rhein-8-O-glucopyranoside,emodin-1-glucoside,chrysophanol-8-glucoside and physcion-8-glucoside were higher than the traditional processing.The content of aloe-emodin-8-O-glucopyranoside and emodin-8-O-glucoside was lower than traditional processing.CONCLUSION The integral processing of rhubarb can reduce effectively the loss of chemical components and ensure the quality of rhubarb pieces.

关 键 词：大黄 饮片 产地加工炮制一体化 传统加工 高效液相色谱法 含量测定 结合蒽醌 游离蒽醌 

分 类 号：R28[医药卫生—中药学]