Global gene expression profiling of blast lung injury of goats exposed to shock wave  

作　　者：Hong Wang Wen-Juan Zhang Jun-Hong Gao Jin-Ren Liu Zhi-Yong Liu Bao-Qing Xia Xiao-Lin Fan Cun-Zhi Li Ai-Rong Qian 

机构地区：[1]Lab for Bone Metabolism,Xi'an Key Laboratory of Special Medicine and Health Engineering,Key Lab for Space Biosciences and Biotechnology,Research Center for Special Medicine and Health Systems Engineering,NPU-UAB Joint Laboratory for Bone Metabolism,School of Life Sciences,Northwestern Polytechnical University,Xi'an 710072,China [2]Research Center for Toxicological and Biological Effects,Institute for Hygiene of Ordnance Industry,Xi'an 710065,China

出　　处：《Chinese Journal of Traumatology》2020年第5期249-257,共9页中华创伤杂志（英文版）

基　　金：This work was supported by Science and Technology Development Fund for Institute for Hygiene of Ordnance(KY202007).

摘　　要：Purpose:Blast lung injury(BLI)is the most common damage resulted from explosion-derived shock wave in military,terrorism and industrial accidents.However,the molecular mechanisms underlying BLI induced by shock wave are still unclear.Methods:In this study,a goat BLI model was established by a fuel air explosive power.The key genes involved in were identified.The goats of the experimental group were fixed on the edge of the explosion cloud,while the goats of the control group were 3 km far away from the explosive environment.After successful modeling for 24 h,all the goats were sacrificed and the lung tissue was harvested for histopathological observation and RNA sequencing.Gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)analysis were performed to identify the main enriched biological functions of differentially expressed genes(DEGs).Quantitative real-time polymerase chain reaction(qRT-PCR)was used to verify the consistency of gene expression.Results:Of the sampled goat lungs,895 genes were identified to be significantly differentially expressed,and they were involved in 52 significantly enriched GO categories.KEGG analysis revealed that DEGs were highly enriched in 26 pathways,such as cytokine-cytokine receptor interaction,antifolate resistance,arachidonic acid metabolism,amoebiasis and bile secretion,JAK-STAT,and IL-17 signaling pathway.Furthermore,15 key DEGs involved in the biological processes of BLI were confirmed by qRTPCR,and the results were consistent with RNA sequencing.Conclusion:Gene expression profiling provide a better understanding of the molecular mechanisms of BLI,which will help to set strategy for treating lung injury and preventing secondary lung injury induced by shock wave.

关 键 词：Blast lung injury Shock wave Differentially expressed genes RNA sequencing TRANSCRIPTOME 

分 类 号：R655.3[医药卫生—外科学]