甘肃省与其他省份育龄妇女维生素D受体基因多态性分布差异性研究  

Study on the distribution difference of vitamin D receptor gene polymorphism between women of childbearing age in Gansu and other provinces

作　　者：许晓英[1] 周敏[1] 王文玲[1] 吴岐珍[1] 靳蕊蕊 李燕婷[1] 杨琳[1] 孟照琰[1] XU Xiao-ying;ZHOU Min;WANG Wen-ling;WU Qi-zhen;JIN Rui-rui;LI Yan-ting;YANG Lin;MENG Zhao-yan(Maternal and Child Health Hospital of Gansu,Lanzhou,Gansu 730050,China)

机构地区：[1]甘肃省妇幼保健院,甘肃兰州730050

出　　处：《现代预防医学》2020年第20期3716-3719,共4页Modern Preventive Medicine

基　　金：甘肃省科技厅自然科学基金(18JR3RA033)。

摘　　要：目的探讨甘肃省育龄期妇女维生素D受体(VDR)基因BsmⅠ及TaqⅠ位点基因多态性与国内其他7省的分布差异。方法采用问卷调查获取全国7个省以及自治区23530名育龄期妇女人口学信息,采集口腔上皮细胞,使用荧光定量PCR技术进行基因型检测,分析甘肃省育龄期妇女维生素D受体(VDR)基因BsmⅠ及TaqⅠ位点基因多态性,并比较甘肃省与其他地区该基因型分布以及等位基因分布的差异。结果甘肃省育龄期妇女维生素D受体(VDR)基因BsmⅠ位点基因型分布为:AA(12,0.40%),AG(329,11.00),GG(2651,88.60%),等位基因A、G的基因频率分别为5.89%、94.11%;TaqⅠ位点基因型分布为:CC(13,0.40%),CT(334,11.20%),TT(2645,88.40%),等位基因C、T的基因频率分别为6.02%、93.98%。与甘肃省育龄期妇女VDR受体BsmⅠ位点基因型分布以及等位基因分布相比,宁夏、云南2省该基因型分布差异具有统计学意义(χ^2=18.568、7.162,P<0.05),宁夏、广东、江苏、云南4省等位基因A、G的基因频率差异具有统计学意义(χ^2=153.723、4.821、6.167、12.430,P<0.05);与甘肃省育龄期妇女VDR受体TaqⅠ位点基因型分布以及等位基因分布相比,各省基因型分布差异不具有统计学意义(P>0.05),云南省等位基因A、G的基因频率差异具有统计学意义(χ^2=5.575,P<0.05)结论甘肃省育龄期妇女VDR基因BsmⅠ及TaqⅠ位点基因多态性与国内其他7省的分布具有明显差异,这可能与地域、生活习惯及民族差异有关。Objective The aim of this study was to investigate the distribution difference of vitamin D receptor(VDR) gene BsmⅠ and TaqⅠ polymorphism between Gansu and seven other provinces. Methods The demographic information of 23530 women of childbearing age in seven provinces of China was obtained by questionnaire survey. The oral epithelial cells were collected, and the genotypes were detected by fluorescence quantitative PCR, in order to analyze the polymorphism of vitamin D receptor(VDR) gene BsmⅠ and TaqⅠ in women of childbearing age in Gansu province, and compare the difference of genotype and allele distribution between Gansu province and other regions. Results In Gansu province, the genotype distribution of vitamin D receptor(VDR) gene BsmⅠ site was AA(12,0.40%), Ag(329,11.00), GG(2651,88.60%), and the gene frequency of allele A and G were 5.89% and 94.11%, respectively. The genotype distribution of TaqⅠ locus was CC(13,0.40%), CT(334,11.20%), TT(2645,88.40%), and the gene frequency of allele C and T was 6.02% and 93. 98%,respectively. Compared with the genotype and allele distribution of VDR receptor BsmⅠ site in women of childbearing age in Gansu province, the difference of genotype distribution between Ningxia and Yunnan was statistically significant(χ~2=18.568,7.162, P<0.05). There were significant differences in allele A and G in Ningxia, Guangdong, Jiangsu and Yunnan(χ~2=153.723,4.821, 6.167, 12.430, P <0.05). Compared with the genotype and allele distribution of VDR receptor Taq Ⅰ site in Gansu province, the differences of genotype distribution in different provinces were not statistically significant(P >0.05). The frequency difference was statistically significant(χ~2=5.575, P<0.05). Conclusion The distribution of VDR gene BsmⅠ and Taq Ⅰ in Gansu province was significantly different from that in seven other provinces, which may be related to regional,living habits and ethnic differences.

关 键 词：维生素D 受体基因多态性 育龄期妇女 甘肃省 

分 类 号：R173[医药卫生—妇幼卫生保健]