冠心病气虚血瘀证病证结合大鼠模型的建立研究  

Study on the establishment of a rat model of coronary heart disease with qi deficiency and blood stasis

作　　者：王瑾茜 蔺晓源[1] 刘侃[1] 胡国恒[2] WANG Jinxi;LIN Xiaoyuan;LIU Kan;HU Guoheng(First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;Hunan University of Traditional Chinese Medicine,Changsha 410208)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]湖南中医药大学,长沙410208

出　　处：《中国实验动物学报》2020年第5期602-609,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81573941);湖南中医药大学校级科研基金(2018XJJJ33);湖南中医药大学中医学国内一流建设学科开放基金(2018ZYX45)。

摘　　要：目的通过分析冠心病(coronary artery heart disease,CHD)气虚血瘀证病证结合大鼠病理、病因、复合模型的舌象表现及与前列环素(prostacyclin,PGI2)、血栓素A2(thromboxane A2,TXA2)的关系,探讨CHD气虚血瘀证病证结合大鼠模型的造模方法。方法选用32只大鼠随机分为4组:K组:正常饮食饮水(n=8);L组:正常饲养21 d后单纯结扎左冠状动脉前降支(n=8);Y组:控食复合疲劳运动21 d(n=8);F组:控食复合疲劳运动21 d+结扎左冠状动脉前降支(n=8)。造模结束后,使用Photoshop 6.0分析舌面RGB值并采用“RGB数据分布范围表”评估各模型舌色;HE染色检测丝状乳头上皮层、角化层、固有层高度及固有层微血管数量;免疫组化分析PGI2、TXA2的阳性表达率并计算T/P比值。结果(1)K组大鼠舌质归属于“淡红舌”;L组大鼠舌面R值较K组显著下降,归属于“暗红舌”;Y组大鼠舌面R值较K组显著下降,归属于“淡白舌”;F组大鼠舌面R、G、B值均显著低于K组和L组,归属于“紫舌”;(2)L组大鼠舌面丝状乳头各层高度、微血管数量与K组间无差异;Y组与K组比较,丝状乳头角化层高度显著降低;F组丝状乳头上皮层、角化层高度均显著低于K组和L组,微血管数量显著高于K组和L组;各组丝状乳头固有层高度均无差异;(3)L组、Y组、F组PGI2变化与K组比较均不明显;L组TXA2水平与T/P比值显著高于K组;F组TXA2水平与T/P比值显著高于K组于L组。结论CHD气虚血瘀证舌色的RGB数值特征与丝状乳头上皮层、角化层高度降低,固有层毛细血管数量增加有关,其分子生物学机制可能与TXA2/PGI2失衡有关;采用病理病因复合模型更符合CHD气虚血瘀证病证结合模型特点。Objective The purpose of this study was to explore a method of coronary heart disease model of qideficiency and blood-stasis syndrome type in rats by comparing the tongue appearance and its relationship with prostacyclin(PGI2)and thromboxane A2(TXA2)of three different model estalishment method,which were respectively established by pathological factors,etiological factors and compoundfactors.Methods Thirty-two rats were randomly divided into four groups:Group K:Normal food and water(n=8);Group L:normal feeding twenty-one days and then ligation the left anterior descending coronary artery(n=8);Group Y:control diet combine with exhautive swimming for twenty-one days(n=8);Group F:control diet combine with exhautive swimming for twenty-one daysand then ligation the left anterior descending coronary artery(n=8).At the end of modeling,Photoshop 6.0 was used to analyze the RGB value of the tongue surface and an RGB data distribution range table was used to evaluate the tongue color of each model.HE staining was used to determine the numbers of microvessels in the upper cortex,keratinized layer,lamina propria,and lamina propria.Immunohistochemistry was used to analyze the positive expression rate of PGI2 and TXA2,and calculate the T/P ratio.Results(1)The tongue quality in Group K was in the category of“light red tongue”.In Group L,the R value of the tongue surface was significantly lower than that in Group K,reflecting“dark red tongue”.In Group Y,the R value of the tongue surface was also significantly lower than that in Group K,reflecting“light white tongue”.In Group F,the R value,G value,and B value of the tongue surface were significantly lower than those in Groups K and L,reflecting“purple tongue”.(2)Compared with Group K,there were no differences in the height of each layer and the number of microvessels of the filiform papilla on the lingual surface in Group L.Compared with Group K,there was a significant decrease in the height of the keratosis layer of filiform papilla in Group Y.In Group F,there w

关 键 词：冠心病 气虚血瘀 病证结合 前列环素 血栓素A2 

分 类 号：Q95-33[生物学—动物学]