左支结扎制备SD大鼠门脉高压动物模型的实验研究  

Experimental study on the impact of ligation of left portal vein on portal vein pressure in SD rats

作　　者：王文晓[1] 柏娟 刘树立[3] WANG Wenxiao;BAI Juan;LIU Shuli(Department of Pediatrics,Yantai Yuhuangding Hospital,Yantai 264000;China.2.Department of Pediatrics,Yantai Yeda Hospital,Yantai 264006;.3.Department of Pediatric Surgery,Capital Institute of Pediatrics,Beijing 100020)

机构地区：[1]烟台毓璜顶医院儿科,山东烟台264000 [2]烟台业达医院儿科,山东烟台264006 [3]首都儿科研究所附属儿童医院普外科,北京100020

出　　处：《中国实验动物学报》2020年第5期675-679,共5页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的探索单纯门静脉左支结扎能否形成稳定的门静脉高压及一氧化氮在其中是否发挥相应的作用。方法36只SPF级雄性8周龄SD大鼠,随机分为左支结扎组(分别用直径0.6、0.7、0.8 mm针结扎),主干结扎组(直径0.9 mm针结扎),左外叶切除组,假手术组共6组。所有大鼠均于术前,术后即刻,术后15 d,术后30 d测量其门脉压力。所有大鼠于术后30 d处死,收集门静脉血液及肝左、右叶组织测量其一氧化氮(NO,nitric oxide)含量。结果左支结扎组SD大鼠术后即刻、15 d、30 d门静脉压力较术前及对照组均明显升高;术后30 d左支0.6 mm组门静脉压力与主干0.9 mm组差异不具有显著性;六组SD大鼠肝左、右叶组织及血液NO含量分别比较均未发现统计学差异。结论单纯门脉左支结扎能形成稳定的门脉高压,直径0.6 mm针能取得较好的结果;NO在肝前型门脉高压中未发挥作用。Objective The aim of the study was to evaluate the relationship between portal hypertension and ligation of left portal vein(LLPV),together with the possible role of NO in prehepatic portal hypertension(PHT).Methods Thirty-six 8-week-old SD rats were divided into six groups:LLPV with needles of 0.6,0.7,or 0.8 mm in diameter;ligation of the main portal vein(LMPV)with a needle of 0.9 mm in diameter;left lateral lobe of liver ligated and removed(LLLR);and sham operation.The portal vein pressure(PVP)was measured in all rats before the operation,immediately after the operation,and 15 and 30 days after the operation.All rats were sacrificed 30 days after the operation.The portal vein blood,and left and right lobes of liver tissues were saved to measure the concentration of NO.Results For rats in the LLPV group,the PVP levels immediately,15 days,and 30 days after the operation were all significantly higher than the preoperative PVP and PVP of the sham operation group at the same time.No statistically significant difference of PVP was found between the LLPV group with a needle of 0.6 mm in diameter and the LMPV group 30 days after the operation.There were also no significant differences of the NO concentration in left and right lobes of liver tissue and blood in all groups.Conclusions LLPV can also form stable PHT.A needle of 0.6 mm in diameter can acquire good result.NO does not play a role in prehepatic PHT.

关 键 词：门脉高压 左支结扎 大鼠 一氧化氮 

分 类 号：Q95-33[生物学—动物学]