CCL4腹腔注射和胆总管结扎致大鼠肝损伤对比的实验性研究  

A Comparative Study of CCl Intraperitoneal Injection and Common Bile Duct Ligation Induced Liver Injury in Rats

作　　者：位小杰 刘东璞[2] 盛延良[2] 贾诗惠 卢凤美[2] Wei Xiaoiie;Liu Dongpu;Sheng Yanliang;Jia Shihui;Lu Fengmei(Graduate School of Jiamusi University,Jiamusi 154003;School of basic medicine,Jiamusi University,Heilongjiang,Jiamusi 154007,China)

机构地区：[1]佳木斯大学研究生院,黑龙江佳木斯154003 [2]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《广东化工》2020年第17期280-281,279,共3页Guangdong Chemical Industry

基　　金：黑龙江省教育厅面上项目编号:2016-KYYWF-0604。

摘　　要：目的:探究CCL4腹腔注射和胆总管结扎(Bile Duct Ligation,BDL)致肝损伤的差异研究。方法:雄性SD大鼠共45只,随机分为正常组(A)、CCL4对照组(B)、BDL对照组(C)、每组5只。CCL4肝损伤组(D),BDL肝损伤(E)每组15只。CCL4模型组(15只)均给予40%CCL4(用花生油2∶3稀释)3m L/kg,2次/周。将D、E两组分别在实验进行1、3、5周断颈处死5只大鼠,取肝脏进行大体观察后取材固定,病理切片观察、免疫组织化学法检测α-SMA、Hab18G/CD147蛋白表达的情况。结果:BDL肝损伤肝脏色黄,主要以胆总管增生,胆汁淤积为主要特征,CCL4肝损伤主要是肝脏表面有脓苔样改变,后期呈暗红色与小肠和膈肌有黏连,与对照组比较,在CCL4肝损伤组中,大鼠肝脏中α-SMA、Hab18G/CD147表达增加,差异有统计学意义(P<0.05);与BDL对照组比较,在BDL肝损伤组中,大鼠肝脏中α-SMA、Hab18G/CD147表达增加,差异有统计学意义(P<0.05)。结论:CCL4与BDL肝损伤有不同特点,在肝损伤过程中α-SMA、Hab18G/CD147的表达随着损伤时间的延长,表达量逐渐增加。Objective: To investigate the difference of liver injury caused by CCl4 intraperitoneal injection and bile duct ligation(BDL). Methods: a total of 45 male SD rats were randomly divided into normal group(a), CCl4 control group(b), BDL control group(c) and 5 rats in each group. CCl4 liver injury group(d), BDL liver injury group(E) 15. The CCl4 model group(15 rats) were given 40 % CCl4(diluted with peanut oil 2∶3) 3 mL/kg twice a week. Five rats in D and e groups were killed at the first, third and fifth week of the experiment respectively. The liver was taken for general observation and fixed, and the expression of α-SMA and HAb18 G/CD147 protein was detected by pathological section and immunohistochemistry. Results: the liver color of BDL liver injury was yellow, which was mainly characterized by choledochal hyperplasia and cholestasis. The liver injury of CCl4 was mainly characterized by purulent changes on the surface of liver, which was later dark red and had adhesion with small intestine and diaphragm. Compared with the control group, in the CCl4 liver injury group, the expression of α-SMA and HAb18 G/CD147 increased significantly(P < 0.05);compared with the BDL control group, the difference was statistically significant(P < 0.05) In BDL group, the expression of α-SMA and HAb18 G/CD147 increased significantly(P < 0.05). Conclusion: the expression of α-SMA and HAb18 G/CD147 in CCl4 is different from that in BDL.

关 键 词：肝损伤 CCL4 BDL HAB18G/CD147 Α-SMA 

分 类 号：TQ[化学工程]