基于网络药理学探讨四妙丸治疗痛风及高尿酸血症作用机制  

Mechanism of Simiao Pill in the Treatment of Gout and Hyperuricemia Based on Network Pharmacology

作　　者：吴越 刘维[1,2] WU Yue;LIU Wei(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Tianjin Key Laboratory of Prescription and Syndrome Translational Research of Traditional Chinese Medicine,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津市中医方证转化研究重点实验室,天津300193

出　　处：《辽宁中医药大学学报》2020年第9期94-100,共7页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81673927,81273709);天津市科技计划(15ZXLCSY00020)。

摘　　要：目的采用网络药理学的方法,对四妙丸治疗痛风及高尿酸血症的作用机制进行研究探讨。方法通过在中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)对四妙丸进行活性成分筛选,运用Cytoscape 3.7.1构建化合物-靶点网络;检索治疗靶点数据库(Therapeutic Target Database,TTD)和DisGenet数据库获取疾病靶标;通过韦恩图,得到疾病与药物的共同靶点;对四妙丸治疗痛风及高尿酸血症的关键靶点进行基因本体(Gene Ontology,GO)功能注释和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析;构建四妙丸化合物-共同靶点-信号通路网络。结果研究显示共挖掘到四妙丸中64种成分及163个潜在靶点,痛风及高尿酸血症165个相关靶点,得到四妙丸-痛风及高尿酸血症共同靶点9个,共同作用靶点主要富集于533个生物过程和20条信号通路上。结论四妙丸可能是通过调节前列腺素内过氧化物合酶2(Prostaglandin-endoperoxide synthase 2,PTGS2)、前列腺素内过氧化物合酶1(Prostaglandin-endoperoxide synthase 1,PTGS1)、肿瘤坏死因子α(tumor necrosis factor,TNF-α)、白介素1β(interlukin-1β,IL-1β)、过氧化物酶体增殖物激活受体γ(peroxisome proliferative activated receptor gamma,PPARG)等靶点,调控破骨细胞分化、c型凝集素受体信号通路、核转录因子κB(nuclear factor kappa-B,NF-κB)信号通路、白介素17(interlukin-17,IL-17)信号通路等通路,从而发挥抗炎、细胞凋亡、细胞周期调控、抗氧化应激来治疗痛风及高尿酸血症。Objective To study the mechanism of Simiao Pill in the treatment of gout and hyperuricemia by means of network pharmacology.Methods The active ingredients of Simiao Pill were screened on TCMSP platform,and the component-target network was constructed by Cytoscape 3.7.1 software;the disease targets were retrieved in TTD and DisGenet databases;the Wein map was constructed to intersect the disease and drug targets,and the therapeutic targets of Simiao Pill for gout and hyperuricemia were obtained.GO function annotation and KEGG pathway enrichment analysis were carried out,and the network of Simiao Pill traditional Chinese medicine ingredient-target-signal pathway was constructed.Results A total of 64 components and 163 potential targets of Simiao Pill,165 related targets of gout and hyperuricemia were found.Nine common targets of Simiao Pill,pain and hyperuricemia,were obtained.The targets of interaction were mainly concentrated in 533 biological processes and 20 signaling pathways.Conclusion Simiao Pill may play an important role in the treatment of gout and hyperuricemia by regulating PTGS2,PTGS1,TNF,IL-1βand PPARG,regulating osteoclast differentiation,C-type lectin receptor signaling pathway,NF-κB signaling pathway,IL-17 signaling pathway and so on.

关 键 词：四妙丸 网络药理学 痛风 高尿酸血症 靶点 信号通路 

分 类 号：R289.5[医药卫生—方剂学]