基于核磁共振技术的人体肺功能障碍疾病血样的代谢组特征  

Metabonomic characteristics of blood serum from patients with chronic obstructive pulmonary disease based on nuclear magnetic resonance

作　　者：何则皓 王桂芳[2] 陈文学[3] HE Zehao;WANG Guifang;CHEN Wenxue(Department of Physics,Fudan University,Shanghai 200433,China;Department of Respiration,Huashan Hospital of Fudan University,Shanghai 200040,China;Department of Chemistry,Fudan University,Shanghai 200433,China)

机构地区：[1]复旦大学物理学系,上海200433 [2]复旦大学附属华山医院呼吸科,上海200040 [3]复旦大学化学系,上海200433

出　　处：《中国医学物理学杂志》2020年第10期1312-1316,共5页Chinese Journal of Medical Physics

基　　金：国家自然科学基金(81673917)。

摘　　要：慢性阻塞性肺病(Chronic Obstructive Pulmonary Disease,COPD)的临床诊断方法主要为肺功能检查和胸部影像学检查(如MRI和CT等),但它们检测的灵敏性和重复性较差。本研究拟通过应用核磁共振氢谱技术检测50例健康者和42例COPD患者血液中一些代谢物的变化,以期在分子水平寻求诊断COPD的生物标志物,以帮助COPD患者进行早期筛查。研究结果发现,在牛磺酸区(δ3.34~3.30)、谷氨酸区(δ2.50~2.26)和脂类区(δ0.90~0.86)等5区域代谢物浓度,以及牛磺酸和谷氨酸区与葡萄糖浓度比值在健康组和COPD组间有显著性差异,此将为COPD诊断标志物的研究提供帮助。Chronic obstructive pulmonary disease(COPD)is mainly diagnosed by lung function test and chest imaging such as MRI and CT,etc.However,the sensitivities and repeatabilities of these diagnostic techniques for COPD diagnosis were unsatisfactory.Herein nuclear magnetic resonance spectroscopy technique is used to analyze the metabonomic differences of serum samples from 50 healthy controls and 42 patients with COPD in order to find the biomarkers for COPD diagnosis at the molecular level,thereby realizing the early screening for COPD in patients.The results showed that there were significant differences between healthy control group and COPD group in the concentrations of some metabolites such as taurine(δ3.34-3.30),glutamate(δ2.50-2.26)and lipid(δ0.90-0.86),and the concentration ratios of taurine and glutamate over glucose,which suggests that the study can provide some reference for the further research on the potential biomarkers for the diagnosis of COPD.

关 键 词：慢性阻塞性肺病 核磁共振 统计分析 生物标志物 

分 类 号：R318[医药卫生—生物医学工程] O657[医药卫生—基础医学]