苦木注射液联用影响阿奇霉素细菌耐药的体外研究  

Antibacterial Activity of Kumu Injection Plus Azithromycin Chemotherapy Against Drug-Resistant Strains by in vitro Evaluation

作　　者：张杰[1] 孙发鑫 郭文娜 许晓蒙 杨昕[2] 许卉[1] ZHANG Jie;SUN Fa-xin;GUO Wen-na;XU Xiao-meng;YANG Xin;XU Hui(School of Pharmacy,Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Key Laboratory of Molecular Pharmacology and Drug Evaluation(Yantai University),Ministry of Education,Yantai University,Yantai 264005,China;School of Chemical and Chemical Engineering in Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学新型制剂与生物技术药物研究山东省高校协同创新中心、分子药理和药物评价教育部重点实验室(烟台大学),山东烟台264005 [2]烟台大学化学化工学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2020年第4期501-504,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目(ZR2017MH061);创新天然药物与中药注射剂国家重点实验室开放课题(QFSKL2018007).

摘　　要：本实验旨在研究阿奇霉素联合苦木注射液对临床常见耐药菌的体外抑菌效果.体外抑菌实验采用96孔微量肉汤稀释法测定标准菌株金黄色葡萄球菌、肺炎链球菌、乙型溶血性链球菌、痢疾志贺菌、肺炎克雷伯菌、大肠埃希氏菌、铜绿假单胞菌的最低抑菌浓度(MIC)及部分抑菌指数(FIC);采用体外药物浓度递增法对联合用药后具协同作用的标准菌株进行诱导耐药,测定两药联合后对高度耐药菌株的MIC及FIC值.结果表明,阿奇霉素联合苦木注射液对标准菌株金黄色葡萄球菌、肺炎链球菌痢疾志贺菌、肺炎克雷伯菌、大肠埃希氏菌具协同抑菌作用,对系列阿奇霉素高度耐药菌均具协同抑菌作用.综上,阿奇霉素联合苦木注射液对临床常见耐药菌有较好的抑菌作用.We make a study on the bacteriostatic effect of azithromycin combined with Kumu injection on clinical common drug-resistant bacteria in vitro.Bacteriostasis experiments of standard strains as Staphylococcus aureus,Streptococcus pneumoniae,α-hemolytic streptococcus,Shigella dysenteriae,Klebsiella pneumoniae,Escherichia coli,Pseudomonas aeruginosa use standard 96-well microbroth dilution method to determine the minimum inhibitory concentration(MIC)and calculate the partial inhibition index(FIC)in vitro.Standard strains with synergistic effects after combination use drug concentrations increasing method to induce drug resistance in vitro,the MIC and FIC values of highly resistant strains are determined after combining the two drugs.The result show that azithromycin combined with Kumu injection has synergistic antibacterial effect on standard strains of S.aureus,S.pneumoniae,S.dysenteriae,K.pneumoniae,E.coli and a series of azithromycin-resistant bacteria,therefore has a good antibacterial infection on clinical common drug-resistant bacteria.

关 键 词：苦木注射液 阿奇霉素 体外诱导耐药 细菌敏感性试验 协同效应 

分 类 号：R917.2[医药卫生—药物分析学]