人参皂苷Rg1对子痫前期大鼠氧化应激和炎症的调控作用及机制研究  

Regulatory effect and mechanism of ginsenoside Rg1 on oxidative stress and inflammation in preeclampsia rats

作　　者：宋姗姗[1] 杨洋[1] 何静[1] 唐俊峰[2] SONG Shanshan;YANG Yang;HE Jing;TANG Junfeng(Obstetrics Department of the First Affiliated Hospital of Xi'an Medical College,Xi'an 710077,Shaanxi,China;Pharmacology Laboratory of Traditional Chinese Medicine,Shaanxi University of Traditional Chinese Medicine,Xianyang 710012,Shaanxi,China)

机构地区：[1]西安医学院第一附属医院产科,陕西西安710077 [2]陕西中医药大学中药药理研究室,陕西咸阳710012

出　　处：《中国临床药理学与治疗学》2020年第10期1088-1096,共9页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：陕西省自然科学基础研究计划项目(2020JM-615)。

摘　　要：目的:探究人参皂苷Rg1对L-亚硝酸左旋精氨酸甲酯(L-NAME)诱导的子痫前期模型大鼠的改善作用及其机制。方法:实验分为4组(n=15):空白组、模型组、人参皂苷Rg1低剂量组(25mg/kg)和高剂量(50 mg/kg)。通过L-NAME诱导建立子痫前期大鼠模型。给药组则在妊娠第5天开始给予相应剂量的人参皂苷Rg1。在第7、13、15、17、19天检查各组实验大鼠的收缩压、舒张压及动脉压;以及测定第20天的24 h尿蛋白。在第21天处死实验大鼠,收集组织样本。采用免疫组化测定胚胎组织中胶质细胞缺失因子1(GCM-1)蛋白;试剂盒测定血清和胚胎组织中的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,以及丙二醛(MDA)、白介素(IL)-1β、IL-6和肿瘤坏死因子(TNF)-α水平变化。Western blot实验检测胚胎组织中Nrf2、HO-1、NF-κB、IκBα、Bcl-2、Bax、Caspase-3、total Cyt-C和胞浆中Cyt-C蛋白表达。结果:实验结果显示,人参皂苷Rg1能够明显改善模型大鼠的收缩压、舒张压、动脉压和尿蛋白升高(P<0.05),抑制胎盘组织中GCM-1、Nrf2、HO-1、Bax、Caspase-3、total Cyt-C和胞浆中Cyt-C蛋白表达降低(P<0.05),促进NF-κB、IκBα和Bcl-2蛋白表达显著性升高(P<0.05);并能够显著性促进模型大鼠血清和胚胎组织中的SOD和CAT活性降低(P<0.05),抑制MDA、IL-1β、IL-6和TNF-α水平升高(P<0.05)。结论:Rg1能够明显改善子痫前期孕鼠的血压和尿蛋白水平,并能够促进其胎盘组织中GCM-1蛋白表达,抑制机体氧化应激损伤、炎症反应,减少胎盘组织细胞凋亡。AIM:To investigate the effect and mechanism of ginsenoside Rg1 on L-NAME-induced preeclampsia in rats.METHODS:All rats were divided into four groups:control group,model group,ginsenoside Rg1 low dose group(25 mg/kg)and high dose group(50 mg/kg).The rat model of preeclampsia was established by L-NAME induction.In the treatment group,the corresponding dose of ginsenoside Rg1 was given at the 5 th day of pregnancy.On the 7 th,13 th,15 th,17 th and 19 th day,the systolic pressure,diastolic pressure and arterial pressure of the experimental rats in each group were examined,and 24-hour urine protein on the 20 th day was measured.The experimental rats were killed on the 21 st day and tissue samples were collected.The protein of GCM-1 was determined by immunohistochemistry,the activities of SOD and CAT,the levels of MDA,IL-1β,IL-6 and TNF-αin serum were determined by kit.Western blot was used to detect the expressions of Nrf2,HO-1,NF-κB,IκBα,Bcl-2,Bax,Caspase-3 and Cyt-c.RESULTS:The results showed that ginsenoside Rg1 could significantly improve the systolic pressure,diastolic pressure,arterial pressure and urinary protein(P<0.05),inhibit the expressions of GCM-1,Nrf2,HO-1,Bax,Caspase-3 and Cyt-c protein in placenta(P<0.05),increase the expressions of NF-κB,IκBαand Bcl-2 protein(P<0.05),and significantly promote the activities of SOD and CAT in serum and fetal tissue of model rats.The levels of MDA,IL-1β,IL-6 and TNF-αwere inhibited(P<0.05).CONCLUSION:Rg1 can significantly improve the blood pressure and urinary protein level of pre-eclampsia pregnant rats,promote the expression of GCM-1 protein in placenta,inhibit oxidative stress injury and inflammatory response,and reduce apoptosis of placenta.

关 键 词：子痫前期 氧化应激 炎症 人参皂苷RG1 

分 类 号：R714.24[医药卫生—妇产科学] R965.2[医药卫生—临床医学]