转录调控因子Lmo2672对LM环境适应性和应激的调控作用  

Regulatory Roles of Lmo2672 Involved in Environmental Adaptability and Stress of Listeria monocytogenes

作　　者：李杰[1] 张星星[2] 孟庆玲[1] 乔军[1] 李妍 王晓婷 李静[1] 才学鹏[3] LI Jie;ZHANG Xing-xing;MENG Qing-ling;QIAO Jun;LI Yan;WANG Xiao-ting;LI Jing;CAI Xue-peng(College of Animal Science and Technology,Shihezi University,Xinjiang Shihezi 832003,China;State Key Laboratory for Sheep Genetic Improvement and Healthy Production,Xinjiang Academy of Agricultural and Reclamation Science,Xinjiang Shihezi 832000,China;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Gansu Lanzhou 730046,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003 [2]新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室,新疆石河子832000 [3]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《西南农业学报》2020年第9期2112-2117,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(31360596,30960274);国家国际科技合作专项(2013DFR31310);省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项(SKLSGIHP2017A03)。

摘　　要：【目的】本文为了解AraC家族转录调控因子Lmo2672对单核增生李斯特菌(LM)环境适应性和应激的影响。【方法】利用温控型质粒pKSV7构建LM-Δlmo2672缺失株,比较母源株LM EGD-e和缺失株LM-Δlmo2672在不同应激环境中生长情况的差异,并利用qRT-PCR方法分析转录调控因子Lmo2672缺失对应激调控基因及氧化应激相关基因转录水平的影响。【结果】与LM EGD-e相比,LM-Δlmo2672在37、42℃及含5%NaCl、0.3%胆酸盐、5 mM H2O2、1%Triton X-100的培养基中生长速度均显著降低(P<0.05);且环境适应性及应激相关基因(prfA、lexA、fri、perR、kat、sod)转录水平显著降低(P<0.05)。【结论】提示lmo2672基因在LM环境适应性和应激中发挥重要的调控作用。【Objective】The present paper aimed to determine the effect of the AraC family transcriptional regulator lmo2672 on the environmental adaptability and stress.【Method】AΔlmo2672 mutant was constructed using temperature-sensitive shuttle plasmid pKSV7,and the environmental adaptability of LM-Δlmo2672 with wild-type strain LM EGD-e,was also compared.Then,the effects of lmo2672 deletion on the transcriptional levels of genes related to stress regulator and oxidative stress of LM using qRT-PCR were explored.【Result】Compared with LM EGD-e,LM-Δlmo2672 grew significantly slowly at 37,42℃,and media containing 5%NaCl,0.3%bile salts,5 mM H2O2,1%Triton X-100(P<0.05).Moreover,the transcription levels of regulatory genes and oxidative stress-related genes(prfA,lexA,fri,perR,kat,sod)were significantly decreased(P<0.05).【Conclusion】These findings indicated that lmo2672 played important regulatory roles in the environmental adaptability and stress.

关 键 词：单核增生李斯特菌 AraC家族 lmo2672基因 环境适应性 

分 类 号：S182[农业科学—农业基础科学]