苦参素对实验性自身免疫性脑脊髓炎大鼠脑内缝隙连接蛋白Cx43和Cx32表达的影响  

Effects of matrine on expressions of Cx43 and Cx32 in brain tissue of experimental autoimmune encephalomyelitis rats

作　　者：楚尧娟[1] 马雯迪 王梦茹 豆萌萌 杜书章[1] 朱琳[1] CHU Yaojuan;MA Wendi;WANG Mengru;DOU Mengmeng;DU Shuzhang;ZHU Lin(Department of Pharmacy, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院药学部,郑州450052

出　　处：《郑州大学学报（医学版）》2020年第5期603-607,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(31870334,31570357)。

摘　　要：目的:研究苦参素对实验性自身免疫性脑脊髓炎(EAE)大鼠脑内缝隙连接蛋白Cx43和Cx32表达的影响。方法:40只雌性Wistar大鼠随机分为正常组、模型组、苦参素治疗组、苦参素预防组4组,每组10只。模型组、苦参素治疗组和预防组大鼠分4点在四肢足垫皮下注射用豚鼠全脊髓匀浆和完全弗氏佐剂制成的抗原乳剂(0.5 mL/只),诱导EAE模型。苦参素预防组于免疫后第1天起腹腔注射苦参素注射液250 mg/kg,1次/d;苦参素治疗组于免疫后第11天起腹腔注射等量的苦参素注射液,1次/d;正常组和模型组于免疫后第1天起腹腔注射等体积生理盐水,1次/d。每天进行神经功能评分。免疫后第18天处死动物,取脑组织,HE和勒克斯光蓝(LFB)染色观察病理组织学改变,免疫荧光法检测脑内Cx43和Cx32蛋白的表达。结果:与正常组比较,模型组大鼠神经功能评分增加,脑组织内有大量的炎性细胞浸润和髓鞘脱失,Cx43蛋白表达水平升高,Cx32表达降低(P均<0.05)。与模型组比较,苦参素治疗组和预防组的神经功能评分降低(P<0.05),脑内炎性细胞浸润和髓鞘脱失减轻(P均<0.05),Cx43蛋白表达水平降低(P<0.05),Cx32蛋白表达升高(P<0.05),且苦参素预防组的作用优于治疗组(P<0.05)。结论:苦参素可能通过调节中枢神经系统(CNS)缝隙连接蛋白Cx43和Cx32的表达,减轻CNS脱髓鞘程度,从而对EAE大鼠起到一定的防治作用。Aim:To investigate the effects of matrine on the expressions of Cx43 and Cx32 in the brain tissue of experimental autoimmune encephalomyelitis(EAE)rats.Methods:Forty female Wistar rats were randomly allocated into four groups:normal group,model group,matrine treatment group and matrine pretreatment group.Antigen emulsion made by homogenate of guinea pig spinal cord and complete Freund′s adjuvant was used to establish EAE model.The rats in the model group,matrine treatment group and matrine pretreatment group were injected subcutaneously at four points in the limb foot pads with 0.5 mL of antigen emulsion.Matrine was given from the 1st day after immunization by intraperitoneal injection daily(250 mg/kg)in the matrine pretreatment group.Matrine was given from the 11th day after immunization by intraperitoneal injection daily(250 mg/kg)in the matrine treatment group.The rats in the normal group and model group were injected normal saline from the 1st day after immunization.The neurological scores were assessed every day.The rats were sacrificed on the 18th day after immunization.HE staining and Luxol fast blue(LFB)staining were carried out in the spinal cord tissue,and the inflammatory infiltration and demyelination were assessed.The expressions of Cx43 and Cx32 in brain tissue were measured by immunofluorescence.Results:Compared with the normal group,the neurological scores were significantly increased,a lot of inflammatory infiltration and demyelination were found in the brain tissue,the expression of Cx43 was significantly increased,and the expression of Cx32 was reduced in the model group(P<0.05).Compared with the model group,the neurological scores were significantly reduced(P<0.05),inflammatory infiltration and demyelination were significantly alleviated(P<0.05),moreover,the expression of Cx43 was markedly down-regulated(P<0.05),and the expression of Cx32 was obviously increased in the matrine treatment group and matrine pretreatment group(P<0.05).The effect of matrine pretreatment group was better than that

关 键 词：苦参素 实验性自身免疫性脑脊髓炎 缝隙连接蛋白 CX43 CX32 

分 类 号：R967[医药卫生—药理学]