盐酸卡替洛尔滴眼液中抑菌剂最适浓度考察及抑菌效力评价  

Studies on the Optimal Bacteriostatic Concentration of Bacteriostat and the Evaluation of Bacteriostatic Efficacy of Carteolol Hydrochloride Eye Drops

作　　者：钱凌 王银环 李小玲 陈枢青[1] QIAN Ling;WANG Yinhuan;LI Xiaoling;CHEN Shuqing(College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China;Zhejiang Institute for Food and Drug Control,Key laboratory of Drug Contacting Materials Quality Control of Zhejiang Provincial,Hangzhou 310052,China;Fuzhou Harvester Pharmaceutical R&D Co.,Ltd.,Fuzhou 350015,China)

机构地区：[1]浙江大学药学院,杭州310058 [2]浙江省食品药品检验研究院,浙江省药品接触材料质量控制研究重点实验室,杭州310052 [3]福州华为医药技术开发有限公司,福州350015

出　　处：《中国现代应用药学》2020年第16期1977-1981,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的探索盐酸卡替洛尔滴眼液中苯扎氯铵的最适添加浓度。方法根据苯扎氯铵药敏试验结果,选择抑菌剂浓度5~70μg·mL-1的盐酸卡替洛尔滴眼液进行初步抑菌效力考察,筛选出适宜的抑菌剂添加量。分别于不同苯扎氯铵浓度的盐酸卡替洛尔滴眼液中加入一定浓度的金黄色葡萄球菌、大肠埃希氏菌、铜绿假单胞菌、白色念珠菌菌悬液和黑曲霉孢子悬液,进行挑战实验,测试上述微生物在不同时间点的存活情况。结果苯扎氯铵40μg·mL-1对5种挑战微生物的生长有良好的抑制作用,且为最低有效剂量。结论苯扎氯铵40μg·mL-1可作为盐酸卡替洛尔滴眼液中抑菌剂的最适添加浓度。OBJECTIVE To investigate the optimal concentration of benzalkonium chloride that added to carteolol hydrochloride eye drops. METHODS According to the minimum inhibitory concentration of benzalkonium chloride. The initial bacteriostatic effect of carteolol hydrochloride eye drops with the concentration of 5–70 mg·mL-1 was investigated, and the appropriate amount of bacteriostatic agent was selected. In addition, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger spore suspension were added to test the survival of the above microorganisms in different periods of time. RESULTS Benzalkonium chloride 40 μg·mL-1 had a good inhibitory effect on the growth of 5 challenging microorganisms and it was the lowest effective dose. CONCLUSION Benzalkonium chloride 40 μg·mL-1 can be used as optimal concentration of bacteriostatic agent in carteolol hydrochloride eye drops.

关 键 词：盐酸卡替洛尔 抑菌剂 苯扎氯铵 抑菌效力 

分 类 号：R927.1[医药卫生—药学]