水稻OsFAH基因启动子的克隆及表达分析  

Cloning and expression analysis of OsFAH promoter in rice

作　　者：胡超[1] 陈彦成 任春梅[1] 张学文[1] 黄丽华[1] HU Chao;CHEN Yancheng;REN Chunmei;ZHANG Xuewen;HUANG Lihua(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley,Ministry of Agriculture,P.R.China,Changsha,Hunan 410125,China;Hunan Rice Research Institute,Changsha,Hunan 410125,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]农业部长江中下游籼稻遗传育种重点实验室,湖南长沙410125 [3]湖南省水稻研究所,湖南长沙410125

出　　处：《湖南农业大学学报（自然科学版）》2020年第5期527-532,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省教育厅项目(18A100);农业部长江中下游籼稻遗传育种重点实验室开放课题(2018KLMA03)。

摘　　要：以湘晚籼13号为材料,克隆了水稻OsFAH基因启动子,构建了OsFAH启动子与GUS基因融合表达载体,并转化拟南芥。序列分析结果表明,该启动子包含了启动子核心序列TATA–box和CAAT–box以及光响应元件等。GUS染色结果表明:在拟南芥幼嫩的子叶、下胚轴和真叶中,GUS的表达较强;随着叶片的衰老,GUS表达减弱;在根中,GUS主要在主根的维管柱内表达;黑暗处理会使GUS表达减弱;在黑暗条件下,OsFAH基因表达下调。The promoter of OsFAH gene was cloned from Oryza sativa cultivar‘Xiangwanxian 13’,and analyzed for the presence of putative cis-elements.The OsFAH promoter contained basal promoter elements,TATA-box,CAAT-box,and the cis-elements involved in light response.The promoter was cloned into a vector that contains the GUS gene,and introduced into Arabidopsis.GUS expression in the transgenic Arabidopsis was detected by histochemical staining.Strong GUS expression was observed in young cotyledons,hypocotyls,and true leaves.However,low GUS expression was only detected in old leaves.GUS expression was found in the vascular tissues of the taproots.And,GUS expression driven by OsFAH promoter and OsFAH expression were decreased when plants were exposed to darkness.

关 键 词：水稻 拟南芥 延胡索酰乙酰乙酸水解酶(FAH) 酪氨酸降解途径 启动子 OsFAH基因 GUS表达 

分 类 号：S511[农业科学—作物学] Q786[生物学—分子生物学]