高效液相色谱法测定人血浆中米卡芬净浓度及其在重症患者中的应用  

Determination of the Concentration of Micafungin in Human Plasma by HPLC and its Application in Critical Patients

作　　者：赵明明[1] 陈亚南[1] 路童 李国飞[1] 肇丽梅[1] ZHAO Mingming;CHEN Yanan;LU Tong;Li Guofei;ZHAO Limei(Dept.of Pharmacy,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院药学部,辽宁沈阳110004

出　　处：《中国医院用药评价与分析》2020年第9期1090-1092,共3页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:建立测定人血浆中米卡芬净浓度的高效液相色谱法。方法:以氯唑沙宗为内标,血浆样品用乙腈沉淀蛋白处理后,经C18色谱柱进行分离,流动相为乙腈-5 mmol/L醋酸铵(V∶V=40∶60),流速为1.0 ml/min,柱温为35℃,检测波长为273 nm,同时进行方法学的验证,并将其应用于重症患者血浆中米卡芬净浓度的测定。结果:米卡芬净在0.2~25.0μg/ml浓度范围内线性关系良好,最低定量下限为0.2μg/ml,低、中及高浓度质量控制样品的回收率分别为88.54%、83.27%及88.16%,日内和日间精密度均在±15%之内。结论:本方法简单快速、准确可靠,可用于临床重症患者米卡芬净血药浓度的监测。OBJECTIVE:To establish a HPLC method for determining the concentration of micafungin in human plasma.METHODS:Chlorzoxazone was set as internal standard substance,the plasma sample was proceeded by acetonitrile protein precipitation and isolated by C18 chromatographic column,with the mobile phase was acetonitrile-5 mmol/L of ammonium acetate(V∶V=40∶60),the flow rate was 1.0 ml/min,the column temperature was 35℃and the detecting wavelength was 273 nm,methodological verification was conducted and applied to determine the concentration of micafungin in human plasma in critical patients.RESULTS:Micafungin was in a good linear relationship in the concentration range of 0.2-25.0μg/ml,with the lowest quantification limit was 0.2μg/ml.The recovery rates of low,medium and high concentration quality control samples were respectively 88.54%,83.27%and 88.16%,the intra-day and inter-day precision were both within±15%.CONCLUSIONS:This method is simple,fast,accurate and reliable,which can be used to monitor the blood concentration of micafungin in critical patients.

关 键 词：米卡芬净 高效液相色谱法 血药浓度 

分 类 号：R978.5[医药卫生—药品]