Determination of Active Components of Lamiophlomis rotata(Benth.)Kudo by Ultra-performance Convergence Chromatography  

作　　者：Guilin JIN Shijun SHAO Fude YANG 

机构地区：[1]University of Tibetan Medicine,Lasa 850000,China [2]Chinese Academy of Sciences,Lanzhou 730000,China [3]Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China

出　　处：《Medicinal Plant》2020年第5期19-22,共4页药用植物：英文版

基　　金：Collaborative Innovation in Tibetan Medicine(2019XTC013);Open Laboratory of Key Laboratory of Tibetan Medicine Basic Education(ZYYJC-19-01).

摘　　要：[Objectives]To determine active components of Lamiophlomis rotata(Benth.)Kudo.[Methods]A new method was developed for the quantitative determination of L.rotata by using Ultra-performance convergence chromatography.The separation of active components of L.rotata was successfully achieved on Waters ACQUITY UPC column,with gradient elution carbon dioxide and methanol solvent at flow rate of 0.7 mL/min.The separation gradient elution rate was as follows:15%methanol 0-1 min,18%methanol 1-5 min,20%methanol 5-6 min,25%methanol 6-7 min,30%methanol 7-8 min,30%methanol 8-9 min,15%methanol 9-10 min.The conditions used in the separation process was as follows:column temperature 30℃;injection volume 2μL.Under the abovementioned conditions,the content of methyl glucoside was measured by 3D-full wavelength scanning.The validity of the method was tested by analyzing the precision,reproducibility,stability and accuracy.[Results]The results indicated that in terms of extraction and separation of compounds,supercritical CO2 not only has the diffusion property of gas,but also has the density and fluidity of liquid.[Conclusions]The ultra-high performance chromatography is a new technique for drug analysis.

关 键 词：Lamiophlomis rotata(Benth.)Kudo Waters UPC2 Active components Content DETERMINATION New method 

分 类 号：O62[理学—有机化学]