Effect of Different Processing Methods on Content ofβ-Asarone in the Zhuang Medicine Rhizoma Acori Tatarinowii  

作　　者：Peng YANG Jiangcun WEI Wen ZHONG Xiumei MA Xuelan LUO Peitao XIE Jiabao MA Qian HAN 

机构地区：[1]Guangxi International Zhuang Medicine Hospital,Nanning 530201,China [2]The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China [3]Guangxi University of Chinese Medicine,Nanning 530200,China [4]Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Nanning 530200,China

出　　处：《Medicinal Plant》2020年第5期56-58,共3页药用植物：英文版

基　　金：Key Research and Development Project of Department of Science and Technology of Guangxi Zhuang Autonomous Region(AB19110003);Project for Improving Basic Scientific Research Ability of Young and Middle-aged Teachers in Colleges and Universities of Guangxi(2019KY0341,2019ky0344);Open Project of Guangxi Zhuang Yao Medicine Center of Engineering and Technology(KJT1900105);Youth Foundation of Guangxi University of Chinese Medicine(2019QN036,2019QN030);Traditional Chinese Medicine Scientific Research Laboratory(Grade III)of National Administration of Traditional Chinese Medicine:Laboratory of Chinese(Zhuang)Medicine Chemical and Quality Analysis(Guo Zhong Yi Yao Fa 2009[21]).

摘　　要：[Objectives]This paper aims to establish a method to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.[Methods]Reversed-phase high-performance liquid chromatography was used,and the chromatographic conditions were as follows:column,Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase,methanol-0.1%phosphoric acid(63∶37);column temperature,30℃;flow rate,1mL/min;detection wavelength,257 nm;sample size,10μL.[Results]The linear range of the injection volume ofβ-asarone was 49.28-246.40μg/mL(R=0.9993);the limit of quantification was 0.85 ng and the detection limit was 0.34 ng;the RSD values of precision,stability and reproducibility tests were all less than 3%;and the sample recovery rate was 98.53%-98.97%(RSD<3.00).The results show that the content ofβ-asarone was highest in shade-dried Rhizoma Acori Tatarinowii.The order ofβ-asarone content was as follows:Rhizoma Acori Tatarinowii dried in shade>Rhizoma Acori Tatarinowii dried at 55℃>Rhizoma Acori Tatarinowii dried at 60℃.[Conclusions]This method is sensitive,reliable,and reproducible.It can be used to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.

关 键 词：Rhizoma Acori Tatarinowii Β-ASARONE Reverse-phase high-performance liquid chromatography Content determination 

分 类 号：TG1[金属学及工艺—金属学]