3株W135群脑膜炎奈瑟菌多位点序列分子分型分析  

Molecular typing of 3 strains of Neisseria meningitidis group W135

作　　者：李曲文 黄梦颖 谢方钦 林震宇 徐海滨 翁顺太 LI Quwen;HUANG Mengying;XIE Fangqin

机构地区：[1]福建省疾病预防控制中心,福建省人兽共患病研究重点实验室,福建医科大学公共卫生学院专业教学实习基地,福建福州350000

出　　处：《实验与检验医学》2020年第5期816-818,共3页Experimental and Laboratory Medicine

基　　金：福建省卫生和计划生育委员会青年科研课题,编号2014-1-26;福建省自然科学基金项目,编号2019J01093。

摘　　要：目的分析我省W135群脑膜炎奈瑟菌(Neisseria meningitides,Nm)分离株的多位点序列分型,了解菌株之间遗传进化关系,为防控流脑提供分子依据。方法通过PCR扩增流脑多位点序列分型(Multilocus Sequence Typing,MLST)的7个管家基因(abcZ、adk、aroE、fumC、gdh、pdhC、pgm),测序分析比对获得序列型(Sequence Type,ST)。结果3株W135群菌株中有2株为ST-11和1株ST-4821,不同型别共存的现象。结论福建省分离的3株W135群Nm存在ST-11和1株ST-4821,应引起对W135流脑的防控重视。Objective The multi-locus sequence typing of neisseria meningitidis isolates from W135 group in our province was analyzed to understand the genetic and evolutionary relationship between the isolates and provide molecular basis for the prevention and control of meningitis.Methods The seven housekeeping genes(abcZ,adk,aroE,fumC,GDH,pdhC,PGM)were amplified by PCR,and the Multilocus Sequence Typing(MLST)was obtained by sequencing analysis.Results Among the 3 strains of W135 group,2 strains were ST-11 and 1 strainST-4821,which showed the coexistence of different types.Conclusion The ST-11 and ST-4821 existed in 3 strains of W135 group Nm isolated from fujian province,which should be paid attention to the prevention and control of W135 encephalitis.

关 键 词：脑膜炎奈瑟菌 W135群 多位点序列分型 

分 类 号：R446.5[医药卫生—诊断学] R372[医药卫生—临床医学]