1,25(OH)2D3/VDR对湿疹患儿miRNA155/SOCS1通路的调节作用  

1,25(OH)2D3/VDR may regulate miRNA155/SOCS1 pathway in the children with eczema

作　　者：江永青 汪红 胡颖 胡志坚 詹忠明 何媛 JIANG Yongqing;WANG Hong;HU Ying;HU Zhijian;ZHAN Zhong-ming;He Yuan(The Hospital of Jiujiang College,Clinical laboratory,Jiangxi.Jiujiang,332000)

机构地区：[1]九江学院附属医院检验科,江西九江332000

出　　处：《实验与检验医学》2020年第5期864-867,共4页Experimental and Laboratory Medicine

基　　金：九江市科技计划项目(九科字〔2017〕28号,项目编号:2017107)。

摘　　要：目的检测湿疹患儿1,25-二羟维生素D3(1,25-dihydroxyvitamin D3,1,25(OH)2D3)、维生素D受体(vitamin D receptor,VDR)、微小核糖核酸155(micro RNA155,miRNA155)、细胞因子信号抑制因子一1(Suppressor of cytokine signaling-1,SOCS1)及淋巴细胞亚群的量,并探讨其相互关系。方法采用电化学发光法检测受试者外周血1,25(OH)2D3的表达,流式细胞术检测患者T细胞百分比及CD3、CD4、CD8阳性细胞含量,采用RT-PCR方法检测皮损处VDR、miRNA155、SOCS1。结果湿疹患儿1,25(OH)2D3及VDR(29.25±11.03 nmol/L,1.74±0.92)明显低于健康儿童(55.49±21.66 nmol/L,3.62±1.68);湿疹患儿T细胞亚群68.65±16.74%及CD4+的T淋巴细胞亚群38.83±10.82%明显高于对照组(64.47±17.23,33.25±11.95%),CD8+的T淋巴细胞亚群25.68±6.81%明显低于对照组29.01±7.93%;湿疹患者皮损处VDR、SOCS的表达(1.74±0.92,1.78±1.02)明显低于对照组(3.62±1.68,3.13±1.70),miRNA1551的表达5.41±3.23明显高于对照组2.06±0.84。结论湿疹患儿1,25(OH)2D3、VDR表达异常,且其可调节湿疹患儿miR155/SOCS1通路。Objective To determine the expression of 1,25(OH)2D3,VDR,miRNA155,SOCS1 and lymphocyte subsets in the children with eczema,and explore their mutual relations.Methods The expression of 1,25(OH)2D3 from eczema patients and healthy controls were examined by pillary electrop,and CD3,CD4,CD8 were detected by flow cytometry.To determine VDR,miRNA155,SOCS1 by RT-PCR.Results In eczema patients,the level of 1,25(OH)2D3 and VDR and SOCS1were greatly lower than in healthy controls.The number of CD3+(%),CD8+(%)were much lower than in the control group,but the number of CD4+(%)was obviously higher in eczema patients.the expression of miRNA155 in eczema lesions was significantly higher than the cases of negative autoantibodies.Conclusion 1,25(OH)2D3 and VDR expression were abnormal in eczema,and they may regulate miRNA155/SOCS1 pathway of the children with eczema.

关 键 词：湿疹 1 25-二羟维生素D3 维生素D受体 微小核糖核酸155 细胞因子信号抑制因子一1 

分 类 号：R446.11[医药卫生—诊断学] R751[医药卫生—临床医学]