尿中苯乙烯代谢产物测定的分散液液微萃取-高效液相色谱法  

Determination of metabolites of styrene in urine by dispersive liquid-liquid microextraction coupled with high performance liquid chromatography

作　　者：顾缨缨 梅勇[1,3] 聂梦涵 盛旭光[1,3] 方若丹 苏婉婷 韩军 Gu Yingying;Mei Yong;Nie Menghan;Sheng Xuguang;Fang Ruodan;Su Wanting;Han Jun(School of Public Health,Wuhan University of Science and Technology,Wuhan 430065,China;College of Resource and Environmental Engineering of Science and Technology,Wuhan 430065,China;Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology,Wuhan 430065,China)

机构地区：[1]武汉科技大学医学院公共卫生学院,430065 [2]武汉科技大学资源与环境工程学院,430065 [3]武汉科技大学,职业危害识别与控制湖北省重点实验室,430065

出　　处：《中华劳动卫生职业病杂志》2020年第9期689-692,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的建立苯乙烯尿中代谢产物苯乙醇酸和苯乙醛酸的分散液液微萃取-高效液相色谱法的测定方法。方法以正辛醇为萃取剂,乙醇为分散剂,对尿中苯乙醇酸和苯乙醛酸进行萃取,涡旋离心后取上层液体,以高效液相色谱仪测定。结果苯乙醇酸在0~10.0 mg/L浓度范围内的线性相关系数>0.999,方法检出限为9.9μg/L,加标回收率为86.1%~101.6%,日内精密度(RSD)为1.07%~3.76%,日间精密度(RSD)为1.24%~3.33%。苯乙醛酸在0.0~2.0 mg/L浓度内的线性相关系数>0.999,方法检出限为2.6μg/L,加标回收率为88.8%~100.3%,日内精密度(RSD)为1.02%~3.17%,日间精密度(RSD)为1.59%~2.41%。结论本方法方法检出限低,富集倍数高,灵敏度好,适用于职业接触苯乙烯人群尿中苯乙醇酸和苯乙醛酸的测定。Objective To establish a method for the determination of mandelic acid and phenylglyoxylic acid in the urine of styrene by dispersive liquid-liquid microextraction-high coupled with high performance liquid chromatography.Methods N-octanol was used as an extractant and ethanol was used as a dispersing agent.The phenylglycolic acid and phenylglyoxylic acid in the urine were extracted,and the upper liquid was taken after vortexing and centrifuged,and then was injected into HPLC for analysis.Results The linear correlation coefficient of the concentration of phenylglycolic acid in the range of 0~10.0 mg/L was greater than 0.999.The detection limit of the method was 9.9μg/L,the recovery rates were 86.1%~101.6%.The intraday RSDs of the method were 1.07%~3.76%,and the interday RSDs were 1.24%~3.33%.The linear correlation coefficient of phenylglyoxylic acid in the range of 0.0~2.0 mg/L is greater than 0.999.The detection limit of the method was 2.6μg/L,the recovery rates were 88.8%~100.3%.The intraday RSDs of the method were 1.02%~3.17%,and the interday RSDs were 1.59%~2.41%.Conclusion The method has low detection limit,high enrichment ratio and good sensitivity,and is suitable for determination of phenylglycolic acid and phenylglyoxylic acid in urine of occupational exposure to styrene.

关 键 词：苯乙醇酸 苯乙醛酸 分散液液微萃取 高效液相色谱 尿 

分 类 号：R446.12[医药卫生—诊断学]