一测多评法测定心可宁胶囊中6种蟾蜍二烯内酯类化合物  

Simultaneous Determination of Six Bufadienolides in Xinkening Capsules with Quantitative Analysis of Multi-components by Single-marker

作　　者：李宗云[1] 刘莉[1] 何世学[1] 赵越 杨美娜[2] 张春宝[1] LI Zongyun;LIU Li;HE Shixue;ZHAO Yue;YANG Meina;ZHANG Chunbao(Harrison International Peace Hospital,Hengshui 053000,China;Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine,Cangzhou 061001,China)

机构地区：[1]哈励逊国际和平医院,河北衡水053000 [2]河北省沧州中西医结合医院,河北沧州061001

出　　处：《中国现代应用药学》2020年第17期2093-2097,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：河北省中医药管理局科研计划项目(2018545);哈励逊国际和平医院研究生科研基金课题项目(2015-06)。

摘　　要：目的建立一测多评法同时测定心可宁胶囊中6种蟾蜍二烯内酯类化合物的含量,并验证此方法在心可宁胶囊中应用的可行性和准确性。方法以华蟾酥毒基为内参物,建立日蟾毒它灵、沙蟾毒精、蟾毒它灵、蟾毒灵和脂蟾毒配基与华蟾酥毒基的相对校正因子。分别采用外标法和一测多评法测定心可宁胶囊中6种蟾蜍二烯内酯类化合物的含量,比较2种方法的结果差异,验证一测多评法的可行性和准确性。结果日蟾毒它灵、沙蟾毒精、蟾毒它灵、蟾毒灵和脂蟾毒配基与华蟾酥毒基的相对校正因子分别为1.06,0.862,0.897,1.09,0.915;2种方法结果无显著差异。结论以华蟾酥毒基为内参物建立的相对校正因子重现性好,一测多评法可用于心可宁胶囊中多个蟾蜍二烯内酯类成分的质量评价。OBJECTIVE To establish quantitative analysis of multi-components by single-marker(QAMS) method for simultaneously determining contents of six bufadienolides in Xinkening Capsules, and to verify the feasibility and accuracy of the method in quality control of Xinkening Capsules. METHODS Cinobufagin was used as the internal reference substance and the relative correlation factors of gamabufotalin, arenobufagin, bufotalin, bufalin and resibufogenin to cinobufagin were calculated. The contents of these six bufadienolides were determined by external standard method and QAMS method respectively, and the difference between the results from the two methods was compared to verify the easibility and accuracy of QAMS method. RESULTS The relative correlation factors were 1.06, 0.862, 0.897, 1.09, 0.915 for gamabufotalin, arenobufagin, bufotalin, bufalin and resibufogenin. There was no significant difference between the quantitative rusults of the QAMS method and the external standard method. CONCLUSION The relative correlation factors established with cinobufagin as the internal reference substance have good reproduciblity. The QAMS method can be used for the quality evaluation of multiple bufadienolides in Xinkening Capsules.

关 键 词：一测多评法 相对校正因子 蟾蜍二烯内酯 心可宁胶囊 

分 类 号：R284.1[医药卫生—中药学]