应用PeriCam PSI血流灌注散斑成像仪对食蟹猴模型舌象的初探研究  

The preliminary study of tongue pictures based on diseases-symptoms in cynomolgus monkey subjects by PeriCam PSI blood flow perfusion speckle imager

作　　者：李舒婷 宗利丽[1] 何伟峰 饶军华 李比海 刘贻颜 刘玉洁[1] 王庭伟 刘凌子 伍庭凤 汪碧云 聂伟哲 LI Shu-ting;ZONG Li-li;HE Wei-feng;RAO Jun-hua;LI Bi-hai;LIU Yi-yan;LIU Yu-jie;WANG Ting-wei;LIU Ling-zi;WU Ting-feng;WANG Bi-yun;NIE Wei-zhe(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Gynecological Diagnosis and Treatment Center of Traditional Chinese Medicine in National Region(South-China Region),Guangzhou 510405,China;First School of Clinic Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Guangdong Institute of Applied Biological Resources,Guangdong Key Laboratory of Animal Conservation and Resource Utilization,Guangdong Public Laboratory of Wild Animal Conservation and Utilization,Guangzhou 510260,China;The Seventh Affiliated Hospital,Sun Yat-sen University,Shenzhen 518000,China;Zhongshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine,Zhongshan 528400,China)

机构地区：[1]广州中医药大学第一附属医院妇儿中心,国家区域(华南区)中医妇科诊疗中心,广州510405 [2]广州中医药大学第一临床医学院,广州510405 [3]广东省生物资源应用研究所,广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室,广州510260 [4]中山大学附属第七医院,广东深圳518000 [5]广州中医药大学附属中山中医院,广东中山528400

出　　处：《中国临床解剖学杂志》2020年第5期578-582,587,共6页Chinese Journal of Clinical Anatomy

基　　金：广州市科技计划项目(No.201607010366)。

摘　　要：目的探索运用PeriCam PSI血流灌注散斑成像仪检测食蟹猴模型舌部血液灌注量的方法并分析其可行性及应用价值。方法(1)5只雌性食蟹猴行宫颈环扎术,制备食蟹猴子宫内膜异位症(endometriosis,EMs)疾病模型,1只雌性食蟹猴开腹仅观察盆腔情况。(2)分别于术前、手术当月及术后1、2、3个月运用PeriCam PSI血流灌注散斑成像仪测量所有食蟹猴舌面、舌底血液灌注量,采用与之配套的PIMSoft软件进行数据采集与分析。结果(1)成功检测6只食蟹猴在不同时间段的舌部血液灌注量,宫颈环扎组食蟹猴术后的血液灌注量均呈现显著上升或下降,差异有统计学意义。(2)术后2月轻-中度盆腔粘连的食蟹猴舌面及舌底的血液灌注量均显著高于无粘连组,差异有统计学意义。结论运用PeriCam PSI血流灌注散斑成像仪测量食蟹猴舌部血液灌注量的方法稳定可行,可能成为疾病病证结合模型舌象的量化评价方法。Objective To explore how to use PeriCam PSI blood perfusion speckle imager to detect the blood perfusion in tongues of cynomolgus monkey subjects and analyze its feasibility and application values. Methods 1. Five female cynomolgus monkeys were subjected to cervical cerclage in preparation for their endometriosis(EMs) models. One of them only served as an indicator of pelvic conditions after its laparotomy. 2. PeriCam PSI blood flow perfusion speckle imager was used to measure the blood perfusion on the surfaces and bases of tongues of all cynomolgus monkeys for their preoperative conditions, in the very month of the coming surgery, and s.p. 1 th, 2 nd and 3 rd months respectively. PIMSoft, the matched software,was used in data collection and analysis. Results 1. Blood perfusion volume on tongues of the 6 subjects at different phases was successfully captured and five out of them s.p. cervical cerclage were seen to undergo significant ups/downs of their blood perfusion volume with statistical significance in their discrepancy. 2. The blood perfusion volume of tongue surfaces and bases of cynomolgus monkeys with s.p. 2 months mildmoderate pelvic adhesion was significantly higher than that of non-adhesion counterparts, the difference being statistically significant. Conclusions The method of PeriCam PSI blood perfusion speckle imager to detectthe blood perfusion in tongues of cynomolgus monkey subjects is stably feasible and might be incorporated in one of the quantitative evaluation methods for modeled tongue pictures based on diseases-symptoms.

关 键 词：PeriCam PSI血流灌注散斑成像仪 舌象 血液灌注量 食蟹猴模型 动物手术 

分 类 号：R711.7[医药卫生—妇产科学]