饱和氢气红细胞保存液对红细胞保存损伤的影响及机制  

Influence of saturated hydrogen red blood cell additive solution on red blood cell storage lesion and its mechanism

作　　者：庞志路 付志杰 王旭 张加强 曹娟 PANG Zhilu;FU Zhijie;WANG Xu;ZHANG Jiaqiang;CAO Juan(Department of Anesthesia and Perioperative Medicine,Henan Prouincial People's Hos pital,ZhengzhouUniversity People's Hospital,Zhengzhou 450003.China;Department of Anesthesiolog,yand Perio perative Medicine,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]河南省人民医院麻醉与围术期医学科郑州大学人民医院,郑州450003 [2]郑州大学第一附属医院麻醉与围术期医学科,郑州450052

出　　处：《中华实用诊断与治疗杂志》2020年第10期993-996,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：河南省科技厅科技攻关项目(172102310101)。

摘　　要：目的探讨饱和氢气红细胞保存液对红细胞保存损伤的影响及可能机制,为提高红细胞保存质量提供新思路。方法 12例健康成年志愿者随机分为饱和氢气组和对照组各6例,2组均采全血50 mL,饱和氢气组加入饱和氢气CAD红细胞保存液,对照组加入CAD红细胞保存液,得到浓缩红细胞悬液。分别于红细胞悬液制备后的第1、10、20、30天检测2组红细胞悬液中红细胞压积(hematocrit, HCT)、K+浓度、红细胞溶血率、游离血红蛋白浓度、超氧化物歧化酶(superoxide dismutase, SOD)活性、过氧化氢酶(catalase, CAT)活性、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)活性、谷胱甘肽(glutathione, GSH)活性以及红细胞膜Na+-K+-ATP酶活性、红细胞膜巯基含量和丙二醛(malondialdehyde, MDA)含量。结果第1天时,2组红细胞悬液HCT、K+浓度、红细胞溶血率、游离血红蛋白浓度、SOD活性、CAT活性、GSH-Px活性、GSH活性以及红细胞膜Na+-K+-ATP酶活性、细胞膜巯基和细胞膜MDA含量比较差异均无统计学意义(P>0.05)。随着红细胞保存时间延长,2组红细胞悬液HCT、SOD活性、CAT活性、GSH-Px活性、GSH活性、Na+-K+-ATP酶活性、细胞膜巯基含量逐渐降低(P<0.05),K+浓度、红细胞溶血率、游离血红蛋白浓度、细胞膜MDA含量逐渐增高(P<0.05);第10、20、30天时,饱和氢气组红细胞悬液HCT、SOD活性、CAT活性、GSH-Px活性、GSH活性以及红细胞膜Na+-K+-ATP酶活性、细胞膜巯基含量明显高于对照组(P<0.05),K+浓度、红细胞溶血率、游离血红蛋白浓度和细胞膜MDA含量明显低于对照组(P<0.05)。结论饱和氢气红细胞保存液可减轻红细胞的保存损伤,可能与饱和氢气红细胞保存液抑制红细胞保存过程中的氧化损伤有关。Objective To investigate the influence and possible mechanism of saturated hydrogen red blood cell additive solution on red blood cell storage lesion to provide a new strategy for improving the quality of red blood cell storage. Methods Twelve healthy adult volunteers were randomly and equally divided into saturated hydrogen group and control group. Each volunteer was collected 50 mL of whole blood. Saturated hydrogen group was added saturated hydrogen CAD red blood cell additive solution, and control group was added CAD red blood cell additive solution to get concentrated red blood cell suspension. On day 1, 10, 20 and 30 after preparation of suspension, the hematocrit(HCT), K^+ concentration, hemolysis rate, free hemoglobin concentration, superoxide dismutase(SOD) activity, catalase(CAT) activity, glutathione peroxidase(GSH-Px) activity, glutathione(GSH) activity, erythrocyte membrane Na^+-K^+-ATPase activity, erythrocyte membrane sulfhydryl content and malondialdehyde(MDA) level were detected in two groups. Results On day 1, there were no significant differences in all indexes between two groups(P>0.05). With the extension of red blood cell storage time, the HCT, SOD activity, CAT activity, GSH-Px activity, GSH, Na^+-K^+-ATPase activity, and cell membrane sulfhydryl content decreased gradually(P<0.05), while the K^+ concentration, red blood cell hemolysis rate, free hemoglobin concentration, and cell membrane MDA content increased gradually(P<0.05). On day 10, 20 and 30, HCT, SOD activity, CAT activity, GSH-Px activity, GSH activity, Na^+-K^+-ATPase activity, and cell membrane sulfhydryl content were significantly higher in saturated hydrogen group than those in control group(P<0.05), and K^+ concentration, red blood cell hemolysis rate, free hemoglobin concentration and cell membrane MDA level were significantly lower than those in control group(P<0.05).Conclusion Saturated hydrogen erythrocyte additive solution can alleviate red blood cell storage lesion,which is probably correlated with the inhibition of ox

关 键 词：红细胞 红细胞保存液 饱和氢气 氧化反应 

分 类 号：R457.1[医药卫生—治疗学]