万古霉素血药浓度快速监测方案的构建与应用  

Construction and Application of Vancomycin Concentrations by HPLC Method

作　　者：王瑞颖 易柳 郭宇鸽[1] 黎旭 高元峰 WANG Rui-ying;YI Liu;GUO Yu-ge;LI Xu;GAO Yuan-feng(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《海峡药学》2020年第9期48-51,共4页Strait Pharmaceutical Journal

基　　金：国家自然基金青年项目基金(No.81803641);湖南省中医药管理局临床药学重点专科(湘中医药函[2017]11号)。

摘　　要：目的建立高效液相色谱(HPLC)快速测定人血浆中万古霉素血药浓度的方法。方法血浆样品经沉淀后,以甲醇-水(含0.05mol·L^-1磷酸二氢钾,pH=3.05)=20∶80为流动相,流速为1.0mL·min^-1,色谱柱为Agilent 5HC-C18(250×4.6mm,5μm),柱温为30℃,紫外检测波长为236nm。结果该法专属性较好,血清中杂质不干扰样品峰的测定;万古霉素在1.0~60.0mg·L^-1质量浓度范围内线性关系良好,所获回归方程为Y=17.68X+5.54(R^2=0.9999);批内、批间精密度和准确度均小于10%。结论HPLC灵敏、快速、准确,操作简便、线性范围宽,可用于万古霉素的临床血药浓度监测。OBJECTIVE To establish a method for determining the concentration of vancomycin in humanplasma by HPLC.METHODS Plasma samples were precipitated with methanol and separation was carried out on a Agilent 5 HC-C18(250×4.6 mm,5μm)at 30℃.The mobile phase consisted of methanol-water(0.05 mol·L^-1 potassium dihydrogen phosphate,pH=3.05)(20∶80)at a flow rate of 1.0 mL·min^-1.The detecting wave length was 236 nm.RESULTS The intrinsic impurity of serum did not interfere with the determination of the analyte.The linearity was good in the concentration range of 1.0-60.0 mg·L^-1 and the linear relationship was Y=17.68 X+5.54(R^2=0.9999).The precision and accuracy within batches and between batches were both less than 10%.CONCLUSION The established HPLC method is fast,sensitive,and accurate,which can be applied to the plasma concentration monitoring of vancomycin.

关 键 词：万古霉素 高效液相色谱法 外标法 临床应用 

分 类 号：R969.1[医药卫生—药理学]