消癌平胶囊中绿原酸和咖啡酸的含量测定  

Determination of Chlorogenic Acid and Caffeic Acid in XiaoAiPing Capsules

作　　者：张敏 刘惠娟 陶梁春 ZHANG Min;LIU Hui-juan;TAO Liang-chun(Gansu Medical college,Gansu University,Pingliang 744000,China)

机构地区：[1]甘肃医学院,甘肃平凉744000

出　　处：《海峡药学》2020年第9期76-77,共2页Strait Pharmaceutical Journal

摘　　要：目的采用高效液相色谱法同时测定消癌平胶囊中绿原酸和咖啡酸的含量。方法采用高效液相法,以Agilent Zorbax SB-C18(5μm,250mm×4.6mm)为色谱柱;流动相:以0.2%磷酸水溶液为流动相A,以乙腈为流动相B;梯度洗脱;流速:1.0mL·min^-1;检测波长为320nm;进样量:20μL。结果绿原酸和咖啡酸在标准曲线范围内呈现良好的线性,精密度、重复性、准确度均符合要求。结论本试验利用高效液相色谱法同时测定消癌平胶囊中绿原酸和咖啡酸的含量,方法学的验证结果表明,该方法准确、可靠、重复性好,可以用于消癌平胶囊中绿原酸和咖啡酸的含量测定。OBJECTIVE To establish a method for the simultaneous determination of chlorogenic acid and caffeic acid in XiaoAiPing capsules by high performance liquid chromatography(HPLC).METHODS HPLC was used with Agilent Zorbax SB-C18(5μm,250 mm×4.6 mm)as chromatographic column;mobile phase:0.2%phosphoric acid aqueous solution as mobile phase A,acetonitrile as mobile phase B;gradient elution;flow rate was 1.0 mL·min^-1;detection wavelength was 320 nm;injection volume was 20μL.RESULTS The linearity of chlorogenic acid and caffeic acid was good in the range of standard curve,and the precision,repeatability and accuracy were all in line with the requirements.CONCLUSION The content of chlorogenic acid and caffeic acid in XiaoAiPing capsules was simultaneously determined by high performance liquid chromatography.The validation results showed that the method was accurate,reliable and reproducible,and could be used for the determination of chlorogenic acid and caffeic acid in XiaoAiPing capsules.

关 键 词：消癌平胶囊 HPLC 绿原酸 咖啡酸 含量测定 

分 类 号：R927[医药卫生—药学]