线粒体靶向载体的合成以及生物活性研究  

Synthesis and Biological Activities of Mitochondrial Targeting Vector

作　　者：王睿 董超 金小红 高涛[2] WANG Rui;JIN Xiao-hong;GAO Tao(School of Pharmacy,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院药学院,湖北咸宁437100 [2]湖北科技学院非动力核技术与化学生物学院

出　　处：《湖北科技学院学报（医学版）》2020年第5期373-376,F0002,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北省自然科学基金(2018CFC822);咸宁市自然科学基金(2019kj07);湖北科技学院科研基金(202022GP02,2018-20XB011)。

摘　　要：目的合成得到新型线粒体靶向载体并研究其生物活性。方法二羰基是常用的金属有机配体,使用吡啶盐-BODIPY修饰二碳基配体,合成得到化合物PBT。通过核磁共振波谱仪,紫外和荧光分光光度计,激光共聚焦显微镜及MTT等仪器和检测方法对化合物进行表征和生物活性研究。结果PBT具有细胞毒性低,生物相容性好,能选择性的聚集于线粒体。结论PBT是具有荧光可视及线粒体靶向功能,并具有较好生物相容性的载体化合物。Objective To synthesize the novel mitochondrial targeting vector and study its biological activities.Methods Mitochondrial targeting vector PBT was synthesized by modification of dicarbonyl with pyridine salt-BODIPY.PBT was characterized by NMR and biological activities were studied by laser confocal microscopy and MTT.Results PBT had low cytotoxicity,good biocompatibility and selective aggregation in mitochondria.Conclusion PBT is an excellent mitochondrial carrier with fluorescence visual function and biocompatibility.

关 键 词：线粒体靶向 吡啶盐-BODIPY 二羰基配体 荧光 

分 类 号：O621.2[理学—有机化学]