基于介孔结构的上转换分子印迹荧光探针检测全氟辛烷磺酸  

Detection of Perfluorooctane Sulfonate by Molecularly Imprinted Upconversion Fluorescent Probe with Mesoporous Structure

作　　者：李晶 郭会琴 于慧 田凌溪 颜流水 刘小明 林立钶 汪嘉琳 LI Jing;GUO Hui-Qin;YU Hui;TIAN Ling-Xi;YAN Liu-Shui;LIU Xiao-Ming;LIN Li-Ke;WANG Jia-Lin(School of Environmental and Chemical Engineering,Nanchang Hangkong University,Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle,Nanchang 330063,China)

机构地区：[1]南昌航空大学环境与化学工程学院,江西省持久性污染物控制与资源循环利用重点实验室,南昌330063

出　　处：《分析化学》2020年第11期1493-1501,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21665018,21966023,21366024);江西省自然科学基金项目(No.20181BAB203014)资助。

摘　　要：全氟辛烷磺酸(Perfluorooctane sulfonate,PFOS)作为环境中最典型的全氟化合物之一,具有持久性、毒性及在生物体内积累等效应。发展快速、灵敏检测环境介质中PFOS的分析方法具有重要意义。本研究以PFOS为模板分子,N,O-双(三甲基硅烷基)三氟乙酰胺为功能单体,设计合成了具有介孔结构的表面分子印迹荧光探针NH 2-UCNPs@MIPs。PFOS可通过F-F与静电相互作用与NH 2-UCNPs@MIPs结合,导致其荧光猝灭,基于此建立了水溶液中PFOS(0.01~15 nmol/L浓度范围内)的高效识别和高灵敏检测技术,并实现了在酸性和中性条件下,对环境水样和人血清基质样品中痕量PFOS的检测。本研究为复杂基质中PFOS的快速检测提供了参考。As one of the most typical perfluorinated compounds in the environment,perfluorooctane sulfonate(PFOS)has the characteristics of persistence,toxicity and accumulation in organisms.It is especially important to establish a rapid and highly sensitive method for detection of PFOS in environmental matrix.In this work,a mesoporous-structured molecularly imprinting fluorescent nanoprobe NH 2-unconversion nanoparticles@molecularly imprinted polymers(NH 2-UCNPs@MIPs)was fabricated with PFOS as template molecule and N,O-bis(trimethylsilyl)trifluoroacetamide as functional monomer.In the analytical process,PFOS could specifically bind amino groups and fluorine-containing groups on the surface of NH 2-UCNPs@MIPs,resulting in the fluorescence quenching of NH [e-mail] on this,a highly selective and sensitive detection of PFOS method was developed with linear range of 0.01-15 nmol/L.The nanoprobe was applied in PFOS detection in surface water and human serum matrix under both acidic and neutral conditions.This work provided a theoretical basis for the rapid and sensitive detection of trace PFOS in complex matrix.

关 键 词：上转换荧光 全氟辛烷磺酸 表面分子印迹 氟-氟相互作用 血清 

分 类 号：O657.3[理学—分析化学] X830.2[理学—化学]