甜菜BvWRKY23基因的RNAi载体构建  

Construction of RNAi Expression Vector of BvWRKY23 Gene in Sugar Beet

作　　者：李国龙[1] 吴海霞 孙亚卿[1] Li Guolong;Wu Haixia;Sun Yaqing(College of Agronomy,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China;Inner Mongolia Research Institute for Water Conservancy,Hohhot 010020,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010018 [2]内蒙古自治区水利科学研究院,内蒙古呼和浩特010020

出　　处：《作物杂志》2020年第5期41-47,共7页Crops

基　　金：国家自然科学基金(31760414,31360355)。

摘　　要：WRKY转录因子家族成员在调控植物生长发育、应答生物与非生物胁迫等方面具有重要的生物学功能。以抗旱甜菜(Beta vulgaris L.)幼苗为材料,提取叶片总RNA并反转录为cDNA,通过RT-PCR方法扩增获得甜菜WRKY转录因子家族成员BvWRKY23基因的RNAi靶片段,以中间载体PBSK-RTM作为媒介,利用传统的"酶切-连接"法构建了含有CaMV 35S启动子、BvWRKY23基因片段反向重复序列的RNAi(RNA interference)植物表达载体pCambia2301ky-BvWRKY23-RNAi。The WRKY transcription factor family members play an important role in regulating plant growth, development and responding to biotic and abiotic stress in plant. In our study, the leaves of sugar beet(Beta vulgaris L.) seedling were used as the material for RNA extraction, the total RNA was extracted and reverse transcribed into cDNA. The RNAi target fragment of BvWRKY23 gene in sugar beet was obtained by RT-PCR amplification. Based on the intermediate vector PBSK-RTM, the RNAi(RNA interference) plant expression vector pCambia2301ky-BvWRKY23-RNAi with inverted repeats of BvWRKY23 driven by the promoter CaMV 35S was successfully constructed by traditional restriction enzyme digestion and ligation methods.

关 键 词：甜菜 WRKY转录因子 RNA干扰 抗旱 

分 类 号：S566.3[农业科学—作物学]