Putative Phosphatase UvPsr1 Is Required for Mycelial Growth, Conidiation, Stress Response and Pathogenicity in Ustilaginonidea virens  

作　　者：Xiong Meng Meng Shuai Qiu Jiehua Shi Huanbin Shen Xiangling Kou Yanjun 

机构地区：[1]Key Laboratory of Three Gorges Regional Plant Genetics&Germplasm Enhancement/Biotechnology Research Center,China Three Gorges University,Yichang 443000,China [2]State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China

出　　处：《Rice science》2020年第6期529-536,共8页水稻科学（英文版）

基　　金：This research was funded by Zhejiang Provincial Natural Science Foundation of China(Grant No.LQ19C140004);Key Research and Development Project of Zhejiang Province(Grant No.2019C02018);the Chinese Academy of Agricultural Sciences under the‘Elite Youth’program,and the Agricultural Sciences and Technologies Innovation Program.We thank Prof.HUANG Junbin of Huazhong Agriculture University(Wuhan,China)for providing inoculum of the U.virens strain HWD-2.

摘　　要：Ustilaginoidea virens is the causal agent of rice false smut,which can be a highly destructive disease of rice.The plasma membrane phosphatase Psr1 proteins,which act as a regulator of the salinity stress response in yeast,are widely distributed across fungi,but their functional characterization is sketchy.In this study,we characterized the functions of Psr1 protein,UvPsr1,in U.virens.Analyses of the AUvpsr1 and its complementation strain showed that UvPsr1 is required for normal mycelial growth,conidiation and tolerance to oxidative,osmotic and cell wall stresses.When rice panicles were inoculated with the AUvpsr1 strains,no symptoms of false smut disease developed,showing that UvPSR1 also contributes to the pathogenicity of the fungus.The deletion mutant of UvPSR1 also appeared to produce a smaller titer of toxic compounds able to inhibit elongation of the germinated seeds.In conclusion,our results indicated that UvPsr1 is a new pathogenic factor of U.virens.

关 键 词：Psr1 rice false smut disease pathogenic factor CLAVICIPITACEAE Ustilaginoidea virens 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]