石荠苧总黄酮对流感病毒性肺炎小鼠肺组织的保护作用  

Protective effect of total flavonoids of ulva lactiflora on lung tissues of mice with influenza virus pneumonia

作　　者：王湘[1] 郭玉芳[1] 王爽[1] WANG Xiang;GUO Yu-fang;WANG Shuang(Department of Pathophysiology,Xi'an Medical College,Xi'an 710021,Shanxi Province,China)

机构地区：[1]西安医学院病理生理教研室,陕西西安710021

出　　处：《中国临床药理学杂志》2020年第20期3224-3227,共4页The Chinese Journal of Clinical Pharmacology

基　　金：陕西省2018年重点研发计划基金资助项目(2018SF-173)。

摘　　要：目的探讨石荠苧总黄酮(TFS)对流感病毒性肺炎小鼠肺组织的保护作用。方法用流感病毒FM1稀释液滴鼻感染的方法诱导构建流感病毒性肺炎小鼠模型,将建立成功的模型小鼠随机分为模型组(n=18)、实验组(n=18)和阳性对照组(n=18),另取18只小鼠予以滴鼻等量0.9%NaCl作为空白组。实验组小鼠予以灌胃TFS(150 mg·kg^-1·d^-1),阳性对照组小鼠予以灌胃利巴韦林(0.02 g·kg^-1·d^-1),空白组与模型组予以灌胃等量0.9%NaCl,连续干预10 d。测定各组小鼠的肺指数;用蛋白免疫印迹法检测肺组织Toll样受体3(TLR3)和β干扰素TIR结构域衔接蛋白(TRIF)蛋白表达情况。结果空白组、模型组、实验组和阳性对照组小鼠肺指数分别为0.67±0.04,1.65±0.31,1.17±0.26,0.89±0.08;TLR3蛋白相对表达量分别为0.15±0.07,0.86±0.14,0.62±0.11,0.31±0.08;TRIF蛋白相对表达量分别为0.29±0.12,0.93±0.07,0.74±0.09,0.42±0.11。模型组与空白组比较,差异均有统计学意义(均P<0.05);实验组、阳性对照组与模型组比较,差异均有统计学意义(均P<0.05)。结论TFS可有效抑制流感病毒性肺炎小鼠炎症反应,同时改善肺组织病理性损伤,保护肺组织,其作用机制可能与抑制肺组织TLR3/TRIF通路的信号转导有关。Objective To investigate the protective effect of total flavonoids of ulva lactiflora(TFS)on lung tissues of mice with influenza virus pneumonia.Methods The mouse model for influenza virus pneumonia were established by nasal infection with FM1 dilution of influenza virus,the successfully established model mice were randomly divided into the model group(n=18),the test group(n=18)and the positive control group(n=18).An additional 18 mice were assigned to the blank group and given an equal amount of 0.9% NaCl.Mice in the test group were given oral administration of TFS(150 mg·kg^-1·d^-1),and mice in the positive control group were given oral administration of ribavirin(0.02 g·kg^-1·d^-1),the model group and the blank group were given oral administration of equal amount of 0.9% NaCl.All groups were treated for 10 d.The lung indexes of each group were measured and calculated;Western blot method was used to detect the expression of Toll-like receptor 3(TLR3)/β-interferon TIR domain adapter protein(TRIF)protein in lung tissues.Results The lung indexes of the blank group,the model group,the test group and the positive control group were 0.67±0.04,1.65±0.31,1.17±0.26,0.89±0.08;the relative expression levels of TLR3 protein in lung tissues were 0.15±0.07,0.86±0.14,0.62±0.11,and 0.31±0.08;the relative expression of TRIF protein were 0.29±0.12,0.93±0.07,0.74±0.09,0.42±0.11.Comparing between model group and blank group,the differences of the factors were significant(all P<0.05);comparing between test group,positive control group and model group,the differences of the factors were significant(all P<0.05).Conclusion TFS can effectively inhibit the inflammatory response of influenza virus pneumonia in mice,at the same time improve the pathological damage of lung tissue,and protect lung tissue,the mechanism may be related to the inhibition of signal transduction of TLR3/TRIF pathway in lung tissue.

关 键 词：流感病毒性肺炎 石荠苧总黄酮 肺指数抑制率 TOLL样受体3 β-干扰素TIR结构域衔接蛋白 

分 类 号：R28[医药卫生—中药学]