北京地区实验大鼠金黄色葡萄球菌的检测结果及MLST分型分析  

Detection Results and MLST Analysis of Staphylococcus aureus in Laboratory Rats from Beijing Area

作　　者：冯育芳 邢进[2] 张雪青[2] 岳秉飞 赵德明[1] FENG Yufang;XING Jin;ZHANG Xueqing;YUE Bingfei;ZHAO Deming(College of Veterinary Medicine,China Agricultural University,Beijing 100094,China;Institute for Laboratory Animal Resources,National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国农业大学动物医学院,北京100094 [2]中国食品药品检定研究院实验动物资源研究所,北京102629

出　　处：《实验动物科学》2020年第4期5-9,共5页Laboratory Animal Science

摘　　要：目的了解北京地区实验大鼠金黄色葡萄球菌的携带情况及分子特征,为实验大鼠金黄色葡萄球菌的防控提供基础信息。方法按照GB/T 14926.14—2001实验动物金黄色葡萄球菌检测方法,对2008—2017年北京地区实验大鼠进行检测,并按年份和季节对检出情况进行分析;然后,对其中主要菌株进行MLST分型及分析。结果2008—2017年在北京地区实验大鼠中检测到金黄色葡萄球菌165株,阳性率为8.44%;2013年达到金葡菌阳性峰值(39株,18.48%),而阳性率在季节间的差异不明显。MLST分型将148株金黄色葡萄球菌分为7个型别(ST8、ST3、ST1、ST6、ST2871、ST88及ST641),其中ST1占比最大(51.35%),其次是ST2871(30.41%),其余ST型别占比都低于10%;进一步分析发现其具有显著的时空分布特点,并存在进化相关性。结论本研究为实验大鼠金黄色葡萄球菌的防控提供了基础信息。Objective To understand the carrying status and molecular characteristics of Staphylococcus aureus in laboratory rats from Beijing area,provide basic information for the prevention and control of Staphylococcus aureus in laboratory rats.Method According to the detection method for Staphylococcus aureus in laboratory animal(GB/T 14926.14—2001),the laboratory rats from Beijing area from 2008 to 2017 were tested,and the detection status was classified by the year and season.Then,the main strains were typed and analyzed by MLST assay.Result From 2008 to 2017,165 strains of Staphylococcus aureus were isolated in laboratory rats from Beijing,with a positive rate of 8.44%.In 2013,the positive peak of Staphylococcus aureus(39 strains,18.48%)was reached,and the positive rate wasn’t significantly different between seasons.MLST classification divided 148 strains of Staphylococcus aureus into 7 types(ST8,ST3,ST1,ST6,ST2871,ST88 and ST641),of which ST1 accounted for the largest proportion(51.35%),followed by ST2871(30.41%).The remaining ST types accounted for less than 10%;further analysis found that it has significant spatial and temporal distribution characteristics,and there is evolutionary correlation.Conclusion This study provides basic information for the prevention and control of Staphylococcusaureus in laboratory rats.

关 键 词：北京地区 实验大鼠 金黄色葡萄球菌 检测结果 MLST分型 

分 类 号：S852.61[农业科学—基础兽医学]