Pictet-Spengler酶的研究进展  

Research progress of Pictet-Spenglerases

作　　者：谢运昌 陈奇 张少飞 申传璞 Yunchang Xie;Qi Chen;Shaofei Zhang;Chuanpu Shen(College of Life Sciences,Jiangxi Normal University,Nanchang 330022,Jiangxi,China;School of Life Sciences,Anhui Medical University,Hefei 230032,Anhui,China;School of Pharmacy,Anhui Medical University,Hefei 230032,Anhui,China;School of Life Sciences,Huaibei Normal University,Huaibei 235000,Anhui,China)

机构地区：[1]江西师范大学生命科学学院,江西南昌330022 [2]安徽医科大学生命科学学院,安徽合肥230032 [3]安徽医科大学药学院,安徽合肥230032 [4]淮北师范大学生命科学学院,安徽淮北235000

出　　处：《生物工程学报》2020年第10期2001-2016,共16页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.41806158,41406195);江西省自然科学基金(No.20202BAB203021);安徽省高校自然科学研究项目(No.KJ2017A177)资助

摘　　要：Pictet-Spengler(P-S)反应通常是指β-芳基乙胺在酸性条件下与醛或者酮发生缩合后环化形成四氢异喹啉或β-咔啉类生物碱结构的一类化学反应。而催化这类高度立体选择性和区域选择性反应的酶称为PictetSpenglerase(P-S酶)。P-S酶是一类重要的活性产物生物合成催化酶,广泛分布于吗啡、那可丁、奎宁、小檗碱、阿吗灵等临床药物以及一些先导化合物的生物合成途径中,应用开发前景广阔。鉴于此,文中对P-S酶的发现、功能鉴定、生物学特性以及催化应用等方面的研究进展进行了归纳总结,以期为P-S酶的后续发掘及系统研究提供良好的借鉴和参考。Pictet-Spenglerases(P-Sases)catalyze the Pictet-Spengler(P-S)reactions and exhibit high stereoselectivity and regioselectivity under mild conditions.The typical P-S reaction refers to the condensation and recyclization ofβ-arylethylamine with aldehyde or ketone under acidic conditions to form tetrahydroisoquinoline andβ-carboline alkaloid derivatives.The related enzymatic products of P-Sases are the backbones of various bioactive compounds,including clinical drugs:morphine,noscapine,quinine,berberine,ajmaline,morphine.Furthermore,the activity of P-Sases in stereoselective and regioselective catalysis is also valuable for chemoenzymatic synthesis.Therefore,this review summarizes the research progress in the discovery,functional identification,biological characteristics and catalytic applications of P-Sases,which provide the useful theoretical reference in future P-Sases research and development.

关 键 词：Pictet-Spengler反应 Pictet-Spengler酶 生物催化 生物碱 生物合成 

分 类 号：Q55[生物学—生物化学]