聚球藻UTEX 2973中光碳驱动的高密度燃料合成  

Light and carbon dioxide-driven synthesis of high-density fuel in Synechococcus elongates UTEX 2973

作　　者：李树斌 孙韬[1,5] 陈磊 张卫文[1,2,3,4,5] Shubin Li;Tao Sun;Lei Chen;Weiwen Zhang(Laboratory of Synthetic Microbiology,School of Chemical Engineering&Technology,Tianjin University,Tianjin 300072,China;Key Laboratory of Systems Bioengineering,Ministry of Education of China,Tianjin 300072,China;Frontier Science Center for Synthetic Biology,Ministry of Education of China,Tianjin 300072,China;Collaborative Innovation Center of Chemical Science and Engineering,Tianjin 300072,China;Center for Biosafety Research and Strategy,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学化工学院合成微生物学实验室,天津300072 [2]教育部系统生物工程重点实验室,天津300072 [3]教育部合成生物学前沿科学中心,天津300072 [4]天津化学化工协同创新中心,天津300072 [5]天津大学生物安全战略研究中心,天津300072

出　　处：《生物工程学报》2020年第10期2126-2138,共13页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31901017);国家重点研发计划(Nos.2019YFA09004600,2018YFA0903600,2018YFA0903000)资助

摘　　要：发展"液态阳光"被认为是解决化石燃料枯竭的关键技术之一。β-石竹烯是高性能的萜烯化合物,作为潜在的航空高密度燃料备受瞩目。新型光合蓝细菌底盘聚球藻UTEX 2973倍增时间短至1.5 h且耐受高温高光,利用光和CO_2合成β-石竹烯具有很大的发展前景。为此,文中在聚球藻UTEX2973中通过构建β-石竹烯合成途径、优化相关关键合酶、增强前体供应等一系列策略实现了在摇瓶中约121.22μg/Lβ-石竹烯的合成(96 h)。在此基础上,通过培养条件的优化实现在光生物反应器中进行高密度培养,最终β-石竹烯产量达到约212.37μg/L(96h)。这是目前报道的蓝细菌底盘中β-石竹烯的最高产量,为未来利用光和CO_2直接合成高密度燃料打下基础。Development of“liquid sunshine”could be a key technology to deal with the issue of fossil fuel depletion.β-caryophyllene is a terpene compound with high energy density and has attracted attention for its potential application as a jet fuel.The high temperature and high light-tolerant photosynthetic cyanobacterium Synechococcus elongatus UTEX 2973(hereafter Synechococcus 2973),whose doubling time is as short as 1.5 h,has great potential for synthesizingβ-caryophyllene using sunlight and CO2.In this study,a production of^121.22μg/Lβ-caryophyllene was achieved at 96 h via a combined strategy of pathway construction,key enzyme optimization and precursor supply enhancement.In addition,a final production of^212.37μg/L at 96 h was realized in a high-density cultivation.To our knowledge,this is the highest production reported forβ-caryophyllene using cyanobacterial chassis and our study provide important basis for high-density fuel synthesis in cyanobacteria.

关 键 词：聚球藻UTEX 2973 高密度燃料 液态阳光 β-石竹烯 高密度培养 

分 类 号：TQ517.2[化学工程] TK6[动力工程及工程热物理—生物能]