HPLC测定酮缬氨酸钙含量的方法优化  

Improvement of Content Determination forα-Ketovaine Calcium by HPLC

作　　者：王宇 朱碧楠 WANG Yu;ZHU Binan(Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China)

机构地区：[1]浙江省食品药品检验研究院,杭州310052

出　　处：《中国现代应用药学》2020年第18期2233-2235,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的优化HPLC测定酮缬氨酸钙含量的方法。方法HPLC采用CAPCELL PAK C18 MGⅡ柱(250 mm×4.6 mm,5μm),流动相为高氯酸钠溶液(pH 2.5)-乙腈(96∶4),柱温为35℃,检测波长205 nm,流速1.0 mL·min-1。结果酮缬氨酸钙在0.025~0.185 mg·mL-1内有良好的线性关系(r=1.0000),平均回收率(n=9)为100.0%。结论该方法简便、快速、准确,灵敏度高,重复性好,可用于酮缬氨酸钙的含量测定。OBJECTIVE To improve an HPLC method for the content determination ofα-ketovaine calcium.METHODS The HPLC method was performed on CAPCELL PAK C18 MGⅡcolumn(250 mm×4.6 mm,5μm)with mobile phase of sodium perchlorate buffer(pH 2.5)-acetonitrile(96∶4).Column temperature was 35℃,detective wavelength was 205 nm,and the flow rate was 1.0 mL·min-1.RESULTS The linear range ofα-ketovaine calcium was 0.025-0.185 mg·mL-1(r=1.0000),the average recovery(n=9)was 100.0%.CONCLUSION The method is simple,rapid and accurate with good repeatability,which can be used for the determination ofα-ketovaine calcium.

关 键 词：酮缬氨酸钙 高效液相色谱法 C18柱 

分 类 号：R917.101[医药卫生—药物分析学]