下丘脑芳香化酶在七氟烷麻醉致新生大鼠癫痫波中的作用  

Role of hypothalamic aromatase in sevoflurane anesthesia-induced epileptic waves in neonatal rats

作　　者：李宁涛[1] 孙铭阳[1] 王婕[1] 丛旭晖 王洋洋 张加强[1] Li Ningtao;Sun Mingyang;Wang Jie;Cong Xuhui;Wang Yangyang;Zhang Jiaqiang(Department of Anesthesiology and Perioperative Medicine,Henan Province People′s Hospital,Zhengzhou 450008,China)

机构地区：[1]河南省人民医院麻醉与围术期医学科,郑州450008

出　　处：《中华麻醉学杂志》2020年第7期805-808,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81771149)。

摘　　要：目的评价下丘脑芳香化酶在七氟烷麻醉致新生大鼠癫痫波中的作用。方法清洁级健康新生SD大鼠30只,雌雄各半,5日龄,体重10~15 g,采用随机数字表法分为3组(n=10):对照组(C组)、七氟烷组(S组)和芳香化酶抑制剂福美司坦+七氟烷组(F组)。监测新生大鼠皮层脑电图(EEG),30 min后F组皮下注射福美司坦2 mg/kg,C组和S组皮下注射生理盐水。皮下给药后30 min时S组和F组吸入6%七氟烷麻醉诱导3 min,随后调整为2.1%麻醉维持57 min。记录七氟烷麻醉期间癫痫波总持续时间、单次持续时间和发作次数;EEG记录结束后剖腹,左心室穿刺取血行血气分析及ELISA法检测皮质酮水平;取脑组织,冰块上迅速分离下丘脑,采用PCR法检测下丘脑芳香化酶mRNA、Na^+-K^+-2Cl^-共同转运体1(NKCC1)mRNA和Na+-K+共同转运体2(KCC2)mRNA的表达。结果C组未见癫痫波。与C组比较,S组和F组皮层癫痫波总持续时间和单次持续时间延长,发作次数增加,血清皮质酮浓度升高,下丘脑芳香化酶mRNA表达上调,NKCC1/KCC2 mRNA比值升高(P<0.05);与S组比较,F组皮层癫痫波总持续时间和单次持续时间缩短,发作次数减少,血清皮质酮浓度降低,下丘脑芳香化酶mRNA表达下调,NKCC1/KCC2 mRNA比值下降(P<0.05)。结论下丘脑芳香化酶表达上调参与了七氟烷麻醉致新生大鼠癫痫波发生的过程。Objective To evaluate the role of hypothalamic aromatase in sevoflurane anesthesia-induced epileptic waves in neonatal rats.Methods Thirty clean-grade healthy neonatal Sprague-Dawley rats of both sexes,aged 5 days,weighing 10-15 g,were divided into 3 groups(n=10 each)according to a random number table method:control group(group C),sevoflurane group(group S),and aromatase inhibitor formestane plus sevoflurane group(group F).The electroencephalogram(EEG)in the neonatal rat cortex was monitored,30 min later formestane 2 mg/kg was subcutaneously injected in F group,while the equal volume of normal saline was given instead in C and S groups.At 30 min after subcutaneous administration,6%sevoflurane was inhaled to induce anesthesia for 3 min,and then the concentration was adjusted to 2.1%to maintain anesthesia for 57 min in S and F groups.The total duration and single duration of epileptic waves and the number of seizure during sevoflurane anesthesia were recorded.After the end of EEG recording,the laparotomy was performed,the left ventricular puncture was performed,and blood samples were collected for blood gas analysis and for determination of corticosterone levels(by enzyme-linked immunosorbent assay).Brain tissues were obtained,and then the hypothalamus was rapidly isolated for determination of the expression of aromatase mRNA,Na^+-K^+-2Cl-cotransporter-1(NKCC1)mRNA and K^+-Cl^-cotransporter-2(KCC2)mRNA(by polymerase chain reaction).Results No epileptic waves were found in group C.Compared with group C,the total duration and single duration of cortical epileptic waves were significantly prolonged,and the number of seizures was increased,the serum corticosterone concentration was increased,the expression of aromatase mRNA was up-regulated,and NKCC1/KCC2 mRNA ratios were increased in S and F groups(P<0.05).Compared with group S,the total duration and single duration of cortical epileptic waves were significantly shortened,and the number of seizures was decreased,the serum corticosterone concentration was decreased,the

关 键 词：芳香酶 下丘脑 麻醉药 吸入 动物 新生 癫痫 

分 类 号：R965[医药卫生—药理学]