CORM-3对创伤性颅脑损伤大鼠输血相关性急性肺损伤的影响  

Effect of CORM-3 on blood transfusion-related acute lung injury in rats with traumatic brain injury

作　　者：张立民[1] 郑维朝 白杨[1] 孙文波[1] 王旭鹏[1] 齐曼曼 李睿[1] Zhang Limin;Zheng Weichao;Bai Yang;Sun Wenbo;Wang Xupeng;Qi Manman;Li Rui(Department of Anesthesiology,Cangzhou Central Hospital,Cangzhou 061001,China)

机构地区：[1]沧州市中心医院麻醉科,061001

出　　处：《中华麻醉学杂志》2020年第7期873-877,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金(81701296);河北省科技厅重点研发计划(172777117)。

摘　　要：目的评价一氧化碳释放分子-3(CORM-3)对大鼠创伤性颅脑损伤后输血相关性急性肺损伤的影响。方法清洁级健康雄性成年SD大鼠72只,体重300~350 g,采用随机数字表法分为4组(n=18):假手术组(S组)、创伤性颅脑损伤组(T组)、创伤性颅脑创伤+10 ml/kg血浆输注组(TP组)和创伤性颅脑创伤+10 ml/kg血浆输注+CORM-3组(TPC组)。采用自由落体法建立大鼠颅脑损伤模型,TP组和TPC组大鼠分别在创伤性颅脑损伤后经股静脉输注血浆10 ml/kg。血浆输注完毕24 h后处死大鼠,取肺组织,确定肺组织湿重/干重(W/D)比值,通过HE染色结果计算肺损伤评分,肺部超声检查进行声像图量化评分,通过TUNEL染色法计算细胞凋亡率,Western blot法检测肺组织活化caspase-3、Bid、Bim和Puma的表达。结果与S组比较,其余3组大鼠肺组织W/D比值、肺损伤评分、声像图量化评分和细胞凋亡率升高,肺组织活化caspase-3、Bid、Bim和Puma表达上调(P<0.05);与T组比较,TP组大鼠肺组织W/D比值、肺损伤评分、声像图量化评分和细胞凋亡率升高,肺组织活化caspase-3、Bid、Bim和Puma表达上调(P<0.05);与TP组比较,TPC组大鼠肺组织W/D比值、肺损伤评分、声像图量化评分和细胞凋亡率下降,肺组织活化caspase-3、Bid和Bim和Puma表达下调(P<0.05)。结论CORM-3可减轻创伤性颅脑损伤大鼠输血相关性急性肺损伤,其机制可能与抑制肺组织细胞凋亡相关。Objective To evaluate the effect of carbon monoxide-releasing molecule-3(CORM-3)on blood transfusion-related acute lung injury in rats with traumatic brain injury(TBI).Methods Seventy-two clean-grade healthy adult male Sprague-Dawley rats,weighing 300-350 g,were divided into 4 groups(n=18 each)using the random number table method:sham operation group(group S),TBI group(T group),TBI plus 10 ml/kg plasma transfusion group(TP group),and TBI plus 10 ml/kg plasma transfusion plus CORM-3 group(TPC group).TBI was induced by dropping a 20-g weight from 20 cm height falling freely in anesthetized rats.Plasma 10 ml/kg was infused via the femoral vein after TBI in TP and TPC groups.The rats were sacrificed at 24 h after plasma transfusion,and lung tissues were obtained for determination of wet/dry weight(W/D)ratio,cell apoptosis,and expression of caspase-3,Bid,Bim and Puma(by Western blot).The lung injury score was calculated using the results of HE staining.Lung ultrasonography was performed for assessment of sonographic score,and the apoptosis rate was calculated by the TUNEL staining method.Results Compared with S group,the W/D ratio,lung injury score,sonographic score and apoptosis rate were significantly increased,and the expression of activated caspase-3,Bid,Bim and Puma was up-regulated in the other three groups(P<0.05).Compared with T group,the W/D ratio,lung injury score,sonographic score and apoptosis rate were significantly increased,and the expression of activated caspase-3,Bid,Bim and Puma was up-regulated in TP group(P<0.05).Compared with TP group,the W/D ratio,lung injury score,sonographic score and apoptosis rate were significantly decreased,and the expression of activated caspase-3,Bid,Bim and Puma was down-regulated in TPC group(P<0.05).Conclusion CORM-3 can reduce acute lung injury related to blood transfusion in rats with TBI,and the mechanism may be related to inhibiting cell apoptosis in lung tissues.

关 键 词：一氧化碳 颅脑损伤 输血 急性肺损伤 细胞凋亡 

分 类 号：R651.15[医药卫生—外科学] R563[医药卫生—临床医学]