补肾益气中药对人绒毛膜外滋养层细胞增殖及分泌相关因子能力的影响  

Effect of Kidney-tonifying and Qi-tonifying Herbs on Proliferation and Secretion of Related Factors of Human Villous Trophoblast Cells

作　　者：龚巍[1] 杜进璇[2] 比丽克孜·艾克木 芦艳丽[1] 万莉[1] 曾斌芳[3] 刘娜[1] GONG Wei;DU Jinxuan;BILIKIZI Ekmu;LU Yanli;WAN Li;ZENG Binfang;LIU Na(Department of Gynecology,The Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830001,Xinjiang,China;Department of Gastroenterology,The Sixth Affiliated Hospital,Xinjiang Medical University,Urumqi 830001,Xinjiang,China;Institute of TCM,Xinjiang Medical University,Urumqi 830001,Xinjiang,China)

机构地区：[1]新疆医科大学第四附属医院妇科,新疆乌鲁木齐830001 [2]新疆医科大学第六附属医院消化科,新疆乌鲁木齐830001 [3]新疆医科大学中医学院,新疆乌鲁木齐830001

出　　处：《中华中医药学刊》2020年第9期33-37,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81160431)。

摘　　要：目的探讨补肾益气中药对人绒毛膜外滋养层细胞(HTR-8)增殖及分泌相关因子能力的影响。方法将60只雌性大鼠随机均分为对照组和补肾益气中药组,补肾益气中药组给予中药煎剂灌胃2 mL/次,早、晚各1次;对照组给予等量的生理盐水灌胃,给药1周后,心脏采血,收集血清。将处于对数生长期的HTR-8细胞做4种处理:空白对照组(含10%对照组大鼠血清的完全培养基)、药低组(含5%补肾益气中药血清的完全培养基)、药中组(含10%补肾益气中药血清的完全培养基)、药高组(含20%补肾益气中药血清的完全培养基)。CCK8法检测HTR-8细胞增殖能力;ELISA试剂盒检测β-HCG分泌水平;PCR检测IGF-2和VEGF的转录表达量。结果含补肾益气中药血清干预后,与对照组比较,不同浓度血清对HTR-8细胞分泌β-HCG量有显著促进作用,差异有统计学意义(P<0.05);虽然随着血清浓度的升高对分泌β-HCG有促进,但不同浓度血清之间比较差异无统计学意义(P>0.05);不同浓度血清对HTR-8细胞IGF-2转录表达量有显著促进作用,差异有统计学意义(P<0.05);药中组与药高组相比较于药低组,对IGF-2转录表达量促进作用更明显,差异有统计学意义(P<0.05),但二组之间差异无统计学意义(P>0.05)。同时,不同浓度血清对HTR-8细胞VEGF转录表达量也有显著促进作用(P<0.05);药高组相比较于药低组和药中组,对VEGF转录表达促进作用更明显,差异有统计学意义(P<0.05),但药低组和药中组之间差异无统计学意义(P>0.05);和对照组相比,中和高浓度血清对HTR-8细胞增殖能力有显著促进作用(P<0.05),但低浓度含药血清干预后虽然增殖能力增强,差异无统计学意义(P>0.05);药中组与药高组相比较于药低组,对细胞增殖促进作用更明显,差异有统计学意义(P<0.05),但二组之间差异无统计学意义(P>0.05)。结论含补肾益气中药血清能促进HTR-8细胞VEGF和IGF-2的表达,使细胞增殖能�Objective To investigate effect of kidney-tonifying and Qi-tonifying herbs on proliferation and secretion of related factors of human villous trophoblast cells(HTR-8).Methods Sixty female rats were randomly divided into control group and kidney-tonifying and Qi-tonifying herbs medicine group.Kidney-tonifying and Qi-tonifying herbalgroup was given Chinese herbal decoction twice a dayin the morning and evening,respectively.The control group was given the same amount of saline by gastric lavage.After a week of administration,the heart bloodand serum were collected.HTR-8 cells in logarithmic growth phase were treated in four ways:blank control group(complete culture medium containing 10%rat serum),low dose group(complete culture medium containing 5%kidney-tonifying and Qi-tonifying serum),middle dosegroup(complete culture medium containing 10%kidney-tonifying and Qi-tonifying serum),high dose group(complete culture medium containing 20%kidney-tonifying and Qi-tonifying serum).CCK8 assay was used to detect the proliferation of HTR-8 cells.ELISA kit was used to detect the secretion ofβ-HCG and qPCR was used to detect the transcriptional expressions of IGF-2 and VEGF.Result After the intervention,compared withthe control group,different concentrations of serum serum containing kidney-tonifying and Qi-tonifying herbssignificantly promoted the secretion ofβ-HCG in HTR-8 cells(P<0.05).Although the secretion ofβ-HCG was promoted with the increase of serum concentration,there was no significant difference among serum of different concentrations(P>0.05).The serum levels of IGF-2 in HTR-8 cells were significantly promoted by different concentrations of serum(P<0.05).Compared with thelow dose group,the middle and high dose groups had a more significant effect on the expression of IGF-2,and the difference was significant(P<0.05),but there was no significant difference between the middleand high dose groups(P>0.05).At the same time,different concentrations of serum containing kidney-tonifying and Qi-tonifyingherbs also signifi

关 键 词：补肾益气中药 HTR-8 Β-HCG IGF-2 VEGF 

分 类 号：R285.5[医药卫生—中药学]