蚕茸柱天胶囊联合维生素E对不育患者精子染色质损伤及氧化应激的影响  

Effect of Canrong Zhutian Capsule combined with vitamin E on sperm chromatin damage and oxidative stress in infertile patients

作　　者：孙军培 杜丹丽[1] 张燕[1] 杨晓东 李雪婷 丁彪 张枫 刘雷 王学故 宫莹莹 Sun Junpei;Du Danli;Zhang Yan;Yang Xiaodong;Li Xueting;Ding Biao;Zhang Feng;Liu Lei;Wang Xuegu;Gong Yingying(Reproductive Medicine Center,The First Affiliated Hospital of Bengbu Medical College,Bengbu 233000,Anhui,China)

机构地区：[1]蚌埠医学院第一附属医院生殖医学中心,安徽蚌埠233000

出　　处：《右江民族医学院学报》2020年第5期592-596,共5页Journal of Youjiang Medical University for Nationalities

基　　金：蚌埠医学院自然科学类项目基金(BYKY1785)。

摘　　要：目的初步探讨蚕茸柱天胶囊联合维生素E对于特发性弱精子症和/或少精子症不育患者精子染色质损伤及氧化应激的临床疗效。方法将60例特发性弱精子症和/或少精子症的男性不育患者随机分为治疗组(n=30)和对照组(n=30),分别予以蚕茸柱天胶囊+维生素E和单纯维生素E治疗90 d,采用计算机辅助精液分析系统(CASA)检测相关精液常规参数,精子染色质扩散法(SCD法)检测精子DNA碎片指数(DFI),抽血查超氧化物歧化酶(SOD)活性,比较治疗前后精子浓度(sperm concentration)、前向运动精子百分率(The percentage of progressively motile sperm rate,PR%)及DFI、SOD活性的变化。结果两组治疗后患者PR及SOD活性明显高于治疗前(P<0.05),DFI明显降低(P<0.05);治疗后治疗组精子浓度明显高于治疗前(P<0.05),而对照组无明显变化(P>0.05)。治疗后治疗组精子浓度、PR及SOD明显高于对照组(P<0.05),DFI明显降低(P<0.05)。治疗组临床总有效率明显高于对照组(P<0.05)。结论蚕茸柱天胶囊联合维生素E治疗对于不育患者精液质量有改善作用,对于精子染色质损伤及氧化应激有一定的改善作用。Objective To preliminarily investigate the clinical effect of Canrong Zhutian Capsule combined with vitamin E on sperm chromatin damage and oxidative stress in infertile patients with idiopathic asthenospermia and/or oligozoospermia.Methods A total of 60 male infertile patients with idiopathic asospermia and/or oligospermia were randomly divided into the treatment group(n=30)and the control group(n=30),and patients in the two groups were treated with Canrong Zhutian Capsule+vitamin E and vitamin E alone for 90 days,respectively.The computer-aided semen analysis system(CASA)was used to detect relevant semen routine parameters.Sperm DNA fragmentation index(DFI)was measured by sperm chromatin diffusion method(SCD).The activity of superoxide dismutase(SOD)was checked by blood examination.Sperm concentration,the percentage of progressively motile sperm(PR%),DFI and SOD activity after treatment were compared with those before treatment.Results After treatment,PR and SOD activity in both groups were significantly higher than those before treatment(P<0.05),with DFI was significantly reduced(P<0.05).After treatment,the sperm concentration in the treatment group was significantly higher than that before treatment(P<0.05),while there was no significant change in the control group(P>0.05).After treatment,sperm concentration,PR and SOD in the treatment group were significantly higher than those in the control group(P<0.05),and DFI significantly reduced(P<0.05).The total clinical effective rate of the treatment group was significantly higher than that of the control group(P<0.05).Conclusion The treatment of Canrong Zhutian Capsule combined with vitamin E can improve the semen quality of infertile patients and improve the sperm chromatin damage and oxidative stress.

关 键 词：不育 染色质 精子 蚕茸柱天胶囊 超氧化物歧化酶 

分 类 号：R711.6[医药卫生—妇产科学]