内生真菌对不同干旱胁迫小花棘豆生理特性影响的研究  

Effects of Endophyte on Physiological Characteristics of Oxytropis glabra under Drought stress

作　　者：鲍根生[1] 陈水红 BAO Gen-sheng(Academy of Animal Science and Veterinary Medicine of Qinghai University,Xining 810016;Key Laboratory of Biological Resources Protection and Utilization in Tarim Basin of Xinjiang Production and Construction Corps,College of Life Science,Tarim University,Alar 843300)

机构地区：[1]青海大学畜牧兽医科学院青海省青藏高原优良种质资源利用重点实验室,西宁810016 [2].新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,塔里木大学生命科学学院,新疆阿拉尔843300

出　　处：《青海畜牧兽医杂志》2020年第5期1-8,共8页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：青海省青藏高原优良种质资源利用重点实验室(2020-ZJ-Y03);国家自然科学基金项目(31660690;31700098)。

摘　　要：小花棘豆是天然草地主要毒害草种类,内生真菌合成的苦马豆素易造成家畜中毒,然而有关内生真菌侵染对非生物逆境下小花棘豆生理响应过程的研究鲜有报道。基于此,本研究以带菌和不带菌小花棘豆为研究对象,测定不同干旱胁迫条件下小花棘豆生理特性。结果表明:随着干旱胁迫程度增加,小花棘豆叶绿素、过氧化物酶、脯氨酸、可溶性糖和可溶性蛋白不断增加,而过氧化氢酶、丙二醛和电导率却持续降低。同时,内生真菌侵染植株的叶绿素、过氧化物酶、脯氨酸、可溶性糖和可溶性蛋白含量显著高于不带菌植株,而过氧化氢酶、丙二醛和电导率却显著低于不带菌植株。可见,内生真菌可显著提高小花棘豆对干旱逆境的耐受能力,进而从侧面支持内生真菌提高小花棘豆适应干旱生境方面的潜在作用。Oxytropis glabra was one of the important toxic weeds and largely distributed in grassland.Swainsonine,one type of metabolites synthesized by endophyte of O.glabra,is attributed to neuropathy disease for herbivores.However,effect of endophyte on physiological responses to drought stress is still unclear.Therefore,the role of endophyte infection on host physiological parameters of O.glabra seedlings were determined undergoing different drought stresses.Chlorophyll,peroxidase activity,proline,soluble sugar and soluble protein were increased with the drought stress intensified;in contrast,malondialdehyde,conductivity and catalase activity were decreased.Comparing with endophyte-free seedlings,endophyte infection has a positive effect to enhance chlorophyll,peroxidase activity,proline,soluble sugar and soluble protein of seedlings,however,malondialdehyde,conductivity and catalase activity of endophyte-infected seedlings were decreased under different drought stresses.Therefore,endophyte infection can enhance host tolerance to drought stress,the role of endophyte should be taken into account host ecological suitability in harsh environments in future research.

关 键 词：内生真菌 苦马豆素 小花棘豆 干旱 生理特性 

分 类 号：S663.9[农业科学—果树学]