Discovery of Simple Sequence Repeat Markers through Transcriptome Analysis of Baccaurea motleyana  

作　　者：Khairun Hisam Nasir Muhammad Fairuz Mohd Yusof Mohd Shahril Firdaus Abdul Razak Siti NorsaidahIbrahim Mira Farzana Mohamad Moktar Khadijah Awang Mohd Shukri Mat Ali 

机构地区：[1]Centre for Molecular Marker Discovery and Validation(CMDV),Agri-Omic dan Bioinformatics,Biotechnology and Nanotechnology Research Centre,Malaysian Agricultural Research and Development Institute(MARDI),Serdang 43400,Selangor,Malaysia [2]Industrial Crop Research Centre,Malaysian Agricultural Research and Development Institute(MARDI),Serdang 43400,Selangor,Malaysia [3]Horticulture Research Centre,Malaysian Agricultural Research and Development Institute(MARDI),Serdang 43400,Selangor,Malaysia

出　　处：《Journal of Food Science and Engineering》2020年第2期33-46,共14页食品科学与工程（英文版）（美国）

摘　　要：Baccaurea motleyana(rambai)is underutilized fruits that are native to Malaysia,Indonesia and Thailand.In this study,a total of 54,779 unigenes identified from rambai transcriptome were used for simple sequence repeat(SSR)analysis by MIcroSAtellite(MISA).A total of 20,420 SSRs were found to be distributed within 37.27%of the total number of unigenes.Mononucleotide repeats represented the main type,accounting for 64.04%,followed by trinucleotide repeats(20.28%)and dinucleotide repeats(19.94%).Gene annotation to seven databases has a success ratio of 68.53%(National Center for Biotechnology Information(NCBI)protein sequences),53.68%(NCBI nucleotide sequences),27.43%(Kyoto Encyclopedia of Genes and Genome Ortholog),56.0%(SwissProt),52.44%(Protein family),53.99%(Gene Ontology)and 26.44%(Kluster of Orthologous Group).Further rambai SSRs were randomly selected and validated to B.motleyana(rambai),B.macrocarpa(tampoi),B.polyneura(jentik-jentik),B.ramiflora(pupor)and B.scortechinii(setambun).

关 键 词：Baccaurea SSR TRANSCRIPTOME gene annotation and SSR validation 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]