系列微生物快速测试瓶技术在长庆油田的应用  

Application of Series Rapid Microbial Bottle Tests Technology in Changqing Oilfield

作　　者：万云洋[1,2] 徐飞艳 王洪关 李文宏 刘利平 郭子龙[5] Wan Yunyang;Xu Feiyan;Wang Hongguan;Li Wenhong;Liu Liping;Guo Zilong(Exploration&Development Research Institute of PetroChina Changqing Oilfield Company,National Engineering Laboratory for Exploration and Development of Low-Permeability Oil&Gas Fields;State Key Laboratory of Petroleum Resources and Prospecting,Research Centre for Geomicrobial Resources and Application,Beijing Key Laboratory of Oil and Gas Pollution Control,China University of Petroleum-Beijing;Beijing Huayou Kelong Development Corporation,China Huayou;Fifth Production Plant,Changqing Oilfield Company,PetroChina;Fourth Production Plant,Changqing Oilfield Company,PetroChina)

机构地区：[1]中国石油大学(北京)油气资源与探测国家重点实验室,地层微生物资源与应用研究中心,油气污染防治北京市重点实验室,非常规油气科学技术研究院 [2]中国石油长庆油田分公司勘探开发研究院,低渗透油气田勘探开发国家工程实验室 [3]中国华油集团北京华油科隆开发公司 [4]中国石油长庆油田分公司第五采油厂 [5]中国石油长庆油田分公司第四采油厂

出　　处：《油气田环境保护》2020年第5期10-12,76,共4页Environmental Protection of Oil & Gas Fields

基　　金：国家科技重大专项(2016ZX05050011,2016ZX05040002);中石油战略合作科技专项(ZLZX2020010805,ZLZX2020020405);中国石油大学(北京)前瞻导向项目(ZX20190209)。

摘　　要：随着微生物技术的快速发展,油田大规模推广微生物技术需要研发适合油田现场和员工操作的便捷方法,测试瓶技术以其实用方便、环保经济的优势成为首选的方法。在油田现场,选取系列纯菌和注入混合样品,规模化实际样品测试由中国石油大学(北京)研制的系列测试瓶,结果表明SRB,DSV,EBX,XXG,PMA,IB系列测试瓶对特征微生物响应性敏感、特异和专一,满足现场无菌条件差、工作量大、快速检测和安全环保的要求。With the rapid development of microbial technology,it is very urgent to develop suitable,rapid and convenient methods for field in-situ and staff operation to match large-scale promotion of microbial technology in oilfield.Bottle test technology for its practical,convenient and environmentally friendly economy has become the preferred method.On the oilfield site,a series samples of pure cultures and injection mixtures were sampled and tested with the test bottles developed by China University of Petroleum(Beijing).The results showed that SRB,DSV,EBX,XXG,PMA and IB series test bottles were sensitive and specific to the characteristic response of specific microorganisms,meeting the requirements of poor asepsis conditions,heavy workload releasing,rapid detection,safety and environmental protection at fields.

关 键 词：测试瓶 长庆油田 纤细竿菌 铜绿假单胞菌 油藏 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]